HIV-1 gag-gp120嵌合基因重组杆状病毒载体的构建  

Construction of recombinant baculovirus vector of HIV-1 gag-gp120 chimeric gene

作　　者：张东威[1] 金宁一[2] 王宏伟[2] 张应玖[3] 王莉馨[4] 罗坤[4] 李萍[2] 于洪臣[4] 殷震[2] 

机构地区：[1]内蒙古通辽市第一人民医院 [2]解放军军需大学研究所病毒室 [3]吉林大学生命科学院 [4]吉林大学预防医学院,吉林长春130021

出　　处：《白求恩医科大学学报》2001年第2期114-116,共3页Journal of Norman Bethune University of Medical Science

基　　金：国家杰出青年基金!资助项目 (3982 5 119)

摘　　要：目的 :构建含有 HIV- 1(中国株 ) gag- gp12 0嵌合基因的重组杆状病毒表达载体。方法 :利用基因重组技术将 B亚型中国株 HIV- 1的结构基因 gag与 gp12 0嵌合后再通过大肠杆菌 /杆状病毒系统构建重组杆状病毒载体。结果 :酶切电泳显示基因重组正确 ,电镜显示重组杆状病毒大量扩增。结论 :含有B亚型 HIV- 1(中国株 ) gag- gp12 0嵌合基因的重组杆状病毒表达载体构建成功 ,为进一步研究Gag- gp12 0嵌合蛋白的表达及其生物学活性奠定了基础。Objective:A new recombinant baculovirus transfer vector was constructed, in which a recombinant gene fragment encoding HIV-1 gag-gp120 chimeric gene was inserted.Methods:After HIV-1 gag gene and gp120 gene were linked,the recombinant baculovirus vector was constructed,and the DNA recombinant technique and the E coli /baculovirus system were used.Results:Gel electrophoresis of DNA analysis showed that the genes were recombined correctly.Electron microscope showed that the recombinant baculovirus reproduced in a great quantity.Conclusion:Recombinant baculovirus vector which HIV-1 gag-gp120 chimeric gene fragment was inserted in was constructed successfully.This vector is useful in study of the expression and the biological function of the HIV-1 Gag-gp120 chimeric protein. [

关 键 词：人I型免疫缺陷病毒 嵌合基因 载体 基因重组 杆状病毒 

分 类 号：R512.91[医药卫生—内科学] Q78[医药卫生—临床医学]