鸡β-防御素-1 SYBR Green Ⅰ实时荧光定量PCR检测方法的建立  

Establishment of SYBR GreenⅠ Real-time Fluorescent Quantitation PCR for Detection of Gallinacin-1

作　　者：李岩[1] 王元心 隋欣[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2014年第6期46-51,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金(30972162)

摘　　要：以鸡β-防御素-1(Gallinacin-1,Gal-1)为研究对象,据GenBank中已发表的鸡β-actin和Gal-1序列设计引物,构建重组质粒作为标准品,成功建立了检测鸡免疫器官组织中Gal-1mRNA表达的实时荧光定量PCR方法,并进行灵敏度、重复性、特异性试验。结果显示,此方法具有快速、高通量、线性范围广、特异性强、灵敏度高等特点,为进一步定量研究Gal-1mRNA表达及其与机体免疫机能的关系奠定了技术基础。The chicken beta-defensin-1 （Gallinacin-1, Gal-1） was regarded as the research object. According to the chicken β-actin and Gal-1 genes sequences published in GenBank, the recombinant plasmids were constructed for the standard, the study successfully established Real-time fluorescence quantitative PCR method for detecting the expression of Gal-1 mRNA in chicken, and tested the sensitivity, repetitiveness and specificity. The results showed that this method was characterized by rapidity, high-flux, wide linear range, good specificity, high sensitivity, and so on. So this method would provide the basis for making further efforts for quantitative research on the expression of Gal-1 mRNA and laying technical foundations for research on the related diseases.

关 键 词：鸡 Gal-1基因 实时荧光定量PCR 

分 类 号：Q78[生物学—分子生物学]