Dab2在马兜铃酸肾病小鼠肾脏中的表达变化  

Expression Changes of Dab2 in Kidney Tissue of the Aristolochic Acid Nephropathy Mice

作　　者：吴金栋[1] 常迪[1] 杨耀晖 田娅慧[1] 赵海焦[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国畜牧兽医》2014年第6期191-196,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家基金(31172362;31372485;31201949);北京农学院2013年科研质量提高经费(高质量论文招标项目);兽医学(中医药)北京市重点实验室;农业应用新技术北京市重点实验室

摘　　要：本试验旨在研究Dab2蛋白在马兜铃酸肾病(aristolochic acid nephropathy,AAN)小鼠模型中的表达变化。在AAN小鼠模型的基础上,利用免疫组化、荧光定量PCR和Western blotting技术对肾脏中Dab2基因转录水平和蛋白表达水平进行检测。结果显示,随着用药时间的延长,肾脏组织损伤程度加重;Dab2蛋白表达量在第6天先降低(P>0.05),在第12天突然升高随后又极显著降低(P<0.01),Dab2蛋白表达总体呈上升趋势,Dab2基因转录水平变化趋势与此一致;同时Dab2蛋白的定位也发生了变化,完成了从刷状缘到细胞质,再到细胞核的转移。结果表明Dab2蛋白参与AAN小鼠的发病过程,为进一步研究Dab2蛋白在AAN中的作用奠定理论基础。The study was aimed to observe the expression changes of Dab2 in kidney tissue of the aristolochic acid nephropathy （ANN） mice. In the foundation of ANN mice model, utilizing the immunohistochemistry, Real-time fluorescence quantitative PCR （qRT-PCR） and Western blotting technology to detect the expression changes of Dab2 in kidney tissues. With the ex tension of treatment time, the kidney structure was more seriously damaged, the expression change of Dab2 decreased on the 6th day （P〉0.05）, suddenly increased on the 12th day and extremely significantly decreased （P〈0.01）, but the overall trend of Dab2 expression change decreased, and the change of mRNA was consistent with this; at the same time, the location of Dab2 proteins changed, transferred from the brush border to the cytoplasm, and transferred into the nucleus of tubular epithelial cells. This study showed that Dab2 involved in the progress of ANN, this study layed the foundation for the function research of Dab2 in the progress of AAN.

关 键 词：Dab2 马兜铃酸肾病 .肾脏 表达 

分 类 号：R692[医药卫生—泌尿科学]