高脂饮食诱导的高胆固醇血症对雄性Wistar大鼠肾脏的毒性作用  

Effect of Diet Induced Hypercholesterolemia on Kidney of Healthy Rats

作　　者：何永成[1] 廖履坦[1] 丁小强[1] 徐元钊[1] 张月娥[1] 韩琴琴 

机构地区：[1]复旦大学附属中山医院 [2]上海市心血管研究所生化室,上海200032

出　　处：《复旦学报（医学版）》2001年第1期42-46,共5页Fudan University Journal of Medical Sciences

基　　金：卫生部科研基金资助项目! (0 78930 1)

摘　　要：目的　研究高胆固醇血症对雄性Wistar大鼠肾脏的毒性作用。方法　用 5 %胆固醇饲料喂养雄性Wistar大鼠 ,制备高胆固醇动物模型 ,分别于实验第 30、6 0和 90d测定肾功能、2 4h尿蛋白、肾皮质胆固醇 (Ch)及各磷脂含量 ,并进行病理形态及组织定量分析。结果　在实验周期内实验组 (E组 )大鼠血浆肌酐水平无显著变化 ;第 90d ,E组 2 4h尿微量白蛋白、肾皮质Ch、磷脂酰胆碱 (PC)及磷脂酰乙醇胺 (PE)显著高于正常对照组(C组 ) ;病理形态及组织定量分析显示肾小球系膜细胞增生、炎性细胞浸润、系膜基质增多、毛细血管塌陷、上皮细胞足突融合 ,肾小球体积增大。IgG直接免疫荧光阴性 ,肾小球内无电子致密物沉积。相关分析显示肾小球体积、肾皮质Ch含量及 2 4h尿微量白蛋白排泄率等与血浆总胆固醇 (TCh)及低密度脂蛋白 (LDL)浓度呈显著正相关关系。Purpose To Investigate the effect of diet induced hyperchole sterolemia on the kidney of Wistar rats. Methods Male Wistar rats were fed with normal chow supplemented with 5%cholesterol and observed biochemical changes in plasma lipid concentration,urinary microalbumin excretion,renal function,lipid component in renal cortices and morphological changes at 30,60 and 90 days. Results Total plasma cholesterol (TCh) and low density lipoprotein (LDL) concentration were significantly elevated in the group E ( P <0.05)at 30 days,and progressively increased thereafter,but during the entire study,there ere no differences in plasma urea nitrogen(BUN),creatinine(Cr),and endogenous creatinine clearance(Ccr) between the two groups.Quantitative urinary microalbumin excration was markely elevated in group E( P <0.05).Cholesterol(Ch),phosphatidylcholine(PC)and phosphatidylethanolamine(PE)levels of the renal cortices were sigificantly increased in the group E at 12 weeks.Progressive development in mesangial hypercellulary,increased mesangial matrix,glomerular capillaries collapes were observed in the group E.No electron dense deposits were observed in any of the glomeruli examined.There was a siginificant positive correlation for the urinary microalbumin,Ch in the renal corticres,and glomerular size with plasma TCh and LDL concentration. Conclusions The diet induced hypercholesterolemia may cause lipid nephrotoxicity in Wistar rats.

关 键 词：高胆固醇血症 脂质肾毒性 磷脂 肾小球系膜基质 WISTAR大鼠 

分 类 号：R589.206[医药卫生—内分泌] R692.02[医药卫生—内科学]