纳米二氧化钛对大鼠NK细胞活性和相关细胞因子的影响  

Effects of titanium dioxide nanoparticles on natural killer cell activity and related cytokines in rat

作　　者：付艳云[1] 张艳秋[1] 马书梅[1] 尹立红[1] 浦跃朴[1] 梁戈玉[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏南京210009

出　　处：《癌变．畸变．突变》2014年第3期161-164,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81172618);国家重大科学研究计划(2011CB9 33404);江苏省青蓝工程项目(2012);江苏省六大人才高峰项目(2013-WSW-053)

摘　　要：目的:研究纳米二氧化钛(TiO2)对大鼠NK细胞活性和相关细胞因子的影响。方法:以SD大鼠为研究模对象,随机分为0.5、4和32 mg/kg 3个纳米TiO2 染毒组,对照组(生理盐水)和微米TiO2染毒组(32 mg/kg)。采用支气管滴注染毒,每周2次,实验持续28 d。分别采用流式细胞仪分析外周血NK细胞数目,乳酸脱氢酶释放法检测脾脏NK细胞活性,液相芯片技术测定血清细胞因子IL-2和IFN-γ含量。结果:随着纳米TiO2染毒剂量的增加,脾脏NK细胞活性逐渐增加,高剂量组NK细胞活性与对照组和微米TiO2组相比, 差别均具有统计学意义(P<0.05)。微米TiO2组与对照组相比,脾脏NK细胞活性无明显变化(P>0.05)。各剂量组外周血NK细胞数目及血清细胞因子IL-2、IFN-γ的变化,与对照组相比差别均无统计学意义(>0.05)。结论:在本实验剂量范围内,纳米TiO2可刺激机体产生免疫应答,增加脾脏NK细胞活性,但NK细胞活性及相关细胞因子的长期变化及潜在的机制仍需要进一步探讨。OBJECTIVE：To explore the effects of titanium dioxide （TiO2） nanoparticles on Natural Killer （NK） cell activity and related cytokines in rats. METHODS：Sprague Dawley rats were randomly divided into 0.5,4 and 32 mg/kg TiO2 nanoparticles exposure groups,control group （0.9%sodium chloride solution）,and TiO2 micro-sized particles exposure group （32 mg/kg）. Rats were treated by intratracheal instillation of these substances in suspension twice a week,a total of 28 consecutive days. NK cell population in peripheral blood was measured by flow cytometry. NK cell killing activity in the spleen was analyzed by lactate dehydrogenase （LDH） release assay. Cytokines IL-2 and INF-γwere measured by liquid phase chips method. RESULTS：The NK cell activity increased with the rise of TiO2 exposure dose. Significant difference in NK cell activity in the spleen was found in the group exposed to 32 mg/kg TiO2 nanoparticles compared with the control group （P〈0.05）. No significant difference was found between control group and micro-sized TiO2 group （P〉0.05）. Compared with micro-sized TiO2 group,NK cell activity was found to be significantly increased in 32 mg/kg TiO2 nanoparticles exposure group （P〈0.05）. No significant change of NK cell populations,IL-2 or INF-γwas observed compared with control group and micro-sized group （P〉0.05）. CONCLUSION：TiO2 nanoparticles could trigger systemic immune responses,and enhance NK cell killing activity in the spleen in the studied dosage range. However,further research is needed to confirm the long-term changes in NK cell populations,NK cell killing activity and related cytokines,as well as the potential mechanisms.

关 键 词：纳米二氧化钛 NK细胞 免疫应答 细胞活性 细胞因子 

分 类 号：Q504[生物学—生物化学] R318.08[医药卫生—生物医学工程]