经环氧乙烷灭菌的培养皿对Ames试验的影响  

Effects of the ethylene oxide-sterilized Petri plate on Ames test

作　　者：张建军[1] 姜凌[1] 张海娇[1] 柴振海[1] 

机构地区：[1]天津药物研究院,天津市新药安全评价研究中心,天津300301

出　　处：《癌变．畸变．突变》2014年第3期228-230,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：十二五"重大新药创制"国家科技重大专项(2012ZX09505001-001)

摘　　要：目的:研究经环氧乙烷灭菌的培养皿对Ames试验的影响。方法:采用Ames试验平板掺入法,分别使用不同储存时间及是否通风等情况下的环氧乙烷灭菌培养皿,计数TA97、TA98、TA100、TA102及TA1535菌株的回变菌落数。结果:灭菌后存放1个月左右(25 d)的培养皿中环氧乙烷的残留在活化和非活化两种条件下均能引起TA100和TA1535菌株的回变菌落数增多(TA100,约自发回变数的1.5倍;TA1535,约自发回变数的10倍),而经通风或延长培养皿储存时间可减少环氧乙烷残留并消除这种致突变作用。结论:在一定条件下,经环氧乙烷灭菌的培养皿对Ames试验中TA100、TA1535菌株有致突变性。OBJECTIVE： Study on effects of the ethylene oxide-sterilized Petri plate on Ames test. METHODS：In the plate incorporation test of Salmonella typhimurium,the revertant colonies of TA97, TA98,TA100,TA102 and TA1535 on the ethylene oxide sterilized Petri plates stored for different periods of time or by ventilation was counted. RESULTS：Ethylene oxide residuals in the Petri plates one month after sterilized increased the revertant colonies of TA100 and TA1535（TA100,about 1.5 fold of the spontaneous revertant colonies;TA1535,about 10 fold of the spontaneous revertant colonies）,and extending storage or ventilation could reduce the plate ethylene oxide residuals to a level that caused no mutagenetic effects. CONCLUSION：Under certain conditions,ethylene oxide-sterilized Petri plate could induce TA100 and TA1535 mutagenicity on Ames test.

关 键 词：环氧乙烷 培养皿 AMES试验 致突变性 

分 类 号：R994.6[医药卫生—毒理学]