APP/PS1双转基因小鼠认知功能和实时步态行为的相关性  

To explore the relationship between the gait behavior changes and cognitive function in APP /PS1 transgenic mice

作　　者：张胜威[1] 董世芬[1] 武汀[1] 靳洪涛[2] 孙建宁[1] 

机构地区：[1]北京中医药大学中药药理系,北京100102 [2]中国医学科学院北京协和医学院药物研究所新药安全评价研究中心,北京100050

出　　处：《中国比较医学杂志》2014年第5期19-24,9,共7页Chinese Journal of Comparative Medicine

基　　金：北京优博项目(YB20101002601);校级自主课题(532/0100604287)

摘　　要：目的初步探讨APP/PS1双转基因小鼠认知功能和实时步态行为学的改变的相关性。方法 3月龄APP/PS1转基因小鼠16只随机分成模型组和石杉碱甲片组,正常对照组选用同龄C57/BL6J小鼠14只。连续治疗150 d,Morris水迷宫(MWM)检测学习记忆能力,大小鼠步态分析仪(GAS-2)检测实时步态行为,并比较两种行为学的相关性。结果与正常对照相比,模型组小鼠有学习记忆能力的显著降低,逃避潜伏期均显著延长(P<0.05),在目标象限停留时间、游泳路程显著降低(P<0.05),第一次穿越平台的时间显著延长(P<0.05),穿台次数显著减少(P<0.05);在步态行为实验中,模型组小鼠平均步行速度显著降低(P<0.05),平均步行周期、绝对值平均体转角和侧向移动均显著升高(P<0.05),在一个步行周期中左后足(left foot,LF)、右后足(right foot,RF)支撑时相显著延长(P<0.05),摆动时相显著缩短(P<0.05),左前足(left hand,LH)、右前足(right hand,RH)、右后足推进指数均显著增加(P<0.05),制动指数显著降低(P<0.05)。石杉碱甲片能显著改善认知功能,并能在一定程度上纠正步态行为的变化。比较两种行为学的相关性,得出学习记忆能力与双前足的制动指数相关性较高(相关系数分别为-0.433、-0.379,P值分别为0.039、0.079),其他方面的相关性不大。结论 APP/PS1转基因小鼠在8月龄时有显著的学习记忆能力障碍和步态行为的改变,并且两种行为学存在一定的相关性。Objective To explore the relationship between the gait behavior changes and cognitive function in APP/PS1 transgenic mice .Methods 16 APP/PS1 transgenic mice were divided into model group and Huperzine A group, C57/BL6J mice with the same age were chosed as control group .After a 150 days consecutive treatment , Morris water maze（MWM） was used to detect the learning and memory ability and Gait analysis system （GAS-2） was used to＆amp;nbsp;detect the gait behavior after the treatment when the mice were 8-month-old.Results The escape latency of the model group was significantly higher than that of the control group （ P 〈0.05 ） , the time spended in the target quadrant , swimming distance in the target quadrant significantly lower than that of the control group （ P 〈0.05 ） , the first time passing through the platform prolonged significantly than the control group （P 〈0.05）, and the number of passing though the platform reduced significantly than the control group （P 〈0.05）.In the gait behavior experiments , compared with the control group, the average walking speed of the model group reduced significantly （P 〈0.05）, the average walking cycle, the absolute average body angle and lateral movement increased significantly （P 〈0.05）;The percentage of support time in a walking cycle of the left and right foot increased significantly （P 〈0.05）.Accordingly, the percentage of swing time in a walking cycle of the left and right foot reduced significantly （P 〈0.05）.The propulsion index of the left hand , right hand, right foot increased significantly （ P 〈0.05）, and then the braking index of the above three feet decreased significantly （ P 〈0.05） .Huperzine A can improve the cognitive function , rectify the changes in the gait behavior .The two behavioral relevance shows that cognitive function and the front two feet braking , propulsion index have a high correlation index （correlation coefficients were -0.433, -0.379, P values were 0.039,0.079）, the oth

关 键 词：APP PS1小鼠 认知功能 实时步态行为 相关性 

分 类 号：R332[医药卫生—人体生理学]