活性数据离散分布下的瑞香狼毒抗肿瘤活性成分筛选研究  

Antitumor components screening of Stellera chamaejasme L. under the case of discrete distribution of active data

作　　者：杨乾栩[1] 程孟春[1] 王莉[1] 阚晓溪[2] 朱晓新[2] 肖红斌[1,2] 

机构地区：[1]中国科学院大连化学物理研究所、中国科学院分离分析化学重点实验室,辽宁大连116023 [2]中国中医科学院中药研究所,北京100700

出　　处：《药学学报》2014年第6期927-931,共5页Acta Pharmaceutica Sinica

基　　金：“重大新药创制”科技重大专项资助项目(2011ZX09307-002-01,2013ZX09301307-004)

摘　　要：研究活性数据离散分布下的瑞香狼毒抗肿瘤活性部位和成分的筛选、提取及验证。分别采用HPD大孔树脂柱和聚酰胺柱获得具有成分差异的瑞香狼毒部位，并用MTT法考察不同部位对人肺癌细胞A549的抑制活性。结果显示，不同部位的A549抑制活性呈现离散分布。权重分析表明，色谱保留时间33．97min成分及30-39min部位对A549抑制作用较大。活性实验表明，30-39rain部位要优于瑞香狼毒醇提物，33．97min成分在100gg·mL-1水平内，与阳性药顺铂作用相当，但作用方式完全不同。在离散活性条件下，权重分析方法对中药活性成分或部位，具有较好的筛选效果。This is to report the screening, extracting and validating antitumor components and compounds from Stellera chamaejasme L. under the case of discrete distribution of active data. In this work, different components from Stellera chamaejasme L. were collected by HPD macroporous resin and polyamide resin column, and their antitumor activity on A549 were tested by MTT assay. Activity results indicate that activity of components at 30-39 min is more potent than that of Stellera chamaejasme L. extract, and the activity of components at 33.97 min is equivalent to positive drug, cis-platinum at 100 μg.mL-l, but with totally different mode of action. Under the case of discrete activity, the weight analysis is capable of screening active components and compounds from natural products.

关 键 词：瑞香狼毒 离散活性数据 权重分析 活性成分 抗肿瘤 

分 类 号：R917[医药卫生—药物分析学]