人血浆中右酮洛芬浓度的HPLC法测定  

Determination of Dexketoprofen in Human Plasma by HPLC

作　　者：贾樱樱 拜年 

机构地区：[1]新疆医科大学第二附属医院,新疆乌鲁木齐830063 [2]新疆医科大学第一附属医院,新疆乌鲁木齐830054

出　　处：《抗感染药学》2014年第2期116-118,共3页Anti-infection Pharmacy

摘　　要：目的：建立 HPLC 法测定人血浆中右酮洛芬的浓度。方法：血浆样品用乙醚作为萃取剂萃取，以萘普生为内标；采用 DiamonsiL C18 色谱柱（5 μm，4.6 mm×200 mm）；流动相为乙腈-5 mol/L 磷酸盐缓冲液（27∶73），pH 值为 6.9，流速为 1.0 mL/min；检测波长为 260 nm。结果：右酮洛芬血浆样品的线性范围为 0.078-5.000 μg/mL（r＝0.999 6）；方法回收率和萃取回收率分别为 99.5%-101.9% 和 70.8%-73.8%（n＝5），日内、日间 RSD 分别为 5.5%-10.8% 和 6.4%-9.4%（n＝5）。结论：本法简便、准确、灵敏度高、专属性强，适用于人血浆中右酮洛芬浓度的测定。Objective：To establish the determination method of the dexketoprofen in Human Plasma by HPLC.Methods： The plasma samples used ether as extractant,and naproxen as internal standard; using DiamonsiL C18 column （5 μm,4.6 mm×200 mm）. Mobile phase was acetonitrile-5 mol/L phosphate buffer （27：73）（pH value=6.9）,flow rate was 1 mL/min and the detection wavelength was 260 nm. Results： The linear range of dexketoprofen plasma sample was 0.078-5.000 μg/mL（r=0.999 6）. The methods recovery and extraction recoveries were betwee 99.5% to 101.9%, and 70.8% to 73.8%（n=5）,respectively. Days and daytime RSD were betwee 5.5% to 10.8%, and 6.4% to 9.4% （n=5）,respectively. Conclusion： The method is simple,accurate,highly sensitive and strongly specific which is suitable for determination of ketoprofen concentration in human plasma.

关 键 词：右酮洛芬 血浆 HPLC法 测定 

分 类 号：R915[医药卫生—微生物与生化药学]