皂角刺抗肿瘤的初步研究  

Preliminary study of Spina Gleditsiae on anti-tumor

作　　者：王莹莹[1] 刘伟杰[1] 杜钢军[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475004

出　　处：《河南大学学报（医学版）》2014年第2期88-90,103,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金项目(81173094);河南省高校青年骨干教师项目(2010GGJS-025);河南省科技厅重点攻关项目(112101310308);河南大学省部共建项目(SBGJ090704)

摘　　要：目的观察皂角刺水煎液的体内外抗肿瘤作用。方法体内实验:建立小鼠背部皮下移植性实体肿瘤H22荷瘤动物模型,灌胃给药后测定肿瘤体积、质量及免疫器官指数;体外实验:用MTT法测定皂角刺对小鼠lewis肺癌细胞的影响。结果体内实验:在本实验剂量下(0.3mL/g),与模型对照组比较,皂角刺组肿瘤生长减缓,抑瘤率为38.37%,胸腺指数、脾脏指数均增高;体外实验:实验所用浓度的皂角刺水煎液呈剂量依赖性抑制小鼠lewis肺癌细胞生长。结论皂角刺有一定抗肿瘤作用,其机制可能与细胞毒和提高机体免疫作用有关。Objective To observe antitumor effects of spina gleditsiae water extract in vitro and in vivo . Methods In vivo ：the subcutaneous model of H22 liver cancer was established to observe antitumor by measuring the volume , weight of in situ tumor and immune organs index after ig of spina gleditsiae water extract . In vitro ：MTT assay is used to test antitumor effects of spina gleditsiae water extract on lewis lung cancer cells . Results In vivo ：compared with model control group , the tumor size in spina gleditsiae group was smaller , the inhibitionory rate was 38 .37% , at the same time , the thymus index and spleen index increased . In vitro ：spina gleditsiae water extract inhibited proliferation in a concentration dependent matter . Conclusion Spina gleditsiae have antitumor activity , its mechanism might be related to cytotoxity and immune modulation .

关 键 词：皂角刺 H22 LEWIS MTT 

分 类 号：R285.5[医药卫生—中药学]