旋毛虫肌幼虫活性鉴别的探讨  

Experimental study on the activity in vitro of muscle larvae of Trichinella spiralis

作　　者：都景芳[1] 刘璋楠 傅皓[2] 秦啸鸣 宋辉[2] 王国英[1] 

机构地区：[1]河南大学医学院细胞与分子免疫学实验室,河南开封475004 [2]河南大学医学院,河南开封475004

出　　处：《河南大学学报（医学版）》2014年第2期97-98,共2页Journal of Henan University:Medical Science

基　　金：河南省教育科学"十二五"规划2013年度立项课题〔(2013)-JKGHC-0166〕

摘　　要：目的通过调节温度鉴别旋毛虫肌幼虫的活性。方法人工消化法收集纯净的肌幼虫,将肌幼虫分别置于3种温度下,观察肌幼虫的形态特点。结果 4℃时,活肌幼虫虫体蜷曲呈螺旋状,活动力弱;37℃时,活肌幼虫虫体呈屈曲样运动,运动活泼;将肌幼虫置100℃温度时,幼虫致死,幼虫虫体呈C字形或虫体内部结构崩解。结论旋毛虫肌幼虫的活虫在4℃时虫体蜷曲,37℃时虫体运动活泼;死亡虫体呈C字形或虫体内部结构崩解。Objective To find a new method that for motion the activity in vitro of muscle larvae of T richinella spiralis by controlling the temperature .Methods The muscle larvae were collected by the artificial digestion protocol . The body characteristics of muscle larvae were observed under different temperature conditions . Results The motion of the muscle larvae was characteristically spiral like at 4℃ but they were more active at 37℃ . The muscle larvae were killed in 100℃ water , their shape became ＂C＂ like and their internal fine structure became disordered . Conclusion The live muscle larvae＇s movement was spiral like at 4℃ and their activity was greater at 37℃ . The dead muscle larvae were＂C＂ shaped and their internal structure was disrupted .

关 键 词：旋毛虫 肌幼虫 活性 鉴别 

分 类 号：R383.1[医药卫生—医学寄生虫学]