干姜对小鼠抗应激能力的影响  

Experiment study on the anti-stress effect of the Dried Ginger extract in mice

作　　者：张旭[1] 游国叶[2] 

机构地区：[1]河南大学药学院,河南开封475004 [2]信阳职业技术学院,河南信阳464000

出　　处：《河南大学学报（医学版）》2014年第2期99-103,共5页Journal of Henan University:Medical Science

基　　金：河南省教育厅自然科学研究指导计划项目(2010B310003);河南大学校内基金(2008YB2R32);开封市科技局项目(100317)

摘　　要：目的研究干姜提取液对小鼠抗应激能力的影响。方法采用强迫游泳法测定小鼠的游泳时间、常压耐缺氧法、热应激及冷应激来测定小鼠的存活时间;通过测定小鼠血清和肝匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性及丙二醛(MDA)的含量,观察干姜提取液对小鼠各种有害刺激的影响。结果干姜提取液在一定浓度连续灌胃(ig)给药14d,能显著延长小鼠的游泳时间(P<0.05或P<0.01)和小鼠常压耐缺氧的存活时间,提高热应激和冷应激小鼠的存活率(P<0.05或P<0.01),肝匀浆中的SOD、CAT活性增加(P<0.05或P<0.01)、MDA水平下降(P<0.05或P<0.01)。结论干姜提取液能增强小鼠的抗疲劳能力、延长常压缺氧小鼠的存活时间、提高低温及高温环境下小鼠的存活率,提高机体对外界应激的反应能力。Objective To observe the anti-stress effect of the Dried Ginger Extract in mice . Methods The effects of aqueous extract and ethanolic extract on the swimming-sustained time , the survival time under hypoxia tolerance , the survival rate by high temperature （ 40 ℃） stimulation and cold temperature（ -6-2 ℃） simulation were observed ,by the method of compelled swimming , anti-hypoxia under normal pressure、40 ℃and -6-2 ℃ respectively . Results The Aqueous extract and Ethanolic extract from dried Ginger could well prolong the swimming time （ P 〈 0 .05 or P 〈 0 .01） ,the survival time of hypoxia and improve the survival rate under the cold temperature（ P 〈 0 .05 or P 〈 0 .01） . Furthermore , after being treated 14d with extract from dried Ginger could significantly increase the activities of superoxide dismutase（SOD） , catalase（CAT ） in liver homogenate and malondialdehyde（ MDA） content was decreased markedly . In addition , the Ethanolic extract from dried Ginger could significantly increase the survival time and the ability of anti-cold temperature .Conclusion the Aqueous extract and Ethanolic extract from dried Ginger could enhance the ability of anti-stress response .

关 键 词：干姜 耐缺氧 抗疲劳 抗高温 耐寒 

分 类 号：R258.6[医药卫生—中医内科学]