量子点荧光免疫渗滤法定量检测血清C反应蛋白的研究  

Study on fluorescent quantum dot immunofiltration assay for quantitative detection of C-reactive protein

作　　者：吴卫华 陈佳[2] 张鹏飞[2] 冉贵萍 

机构地区：[1]上海奉贤区奉城医院检验科,上海201411 [2]上海长征医院转化医学中心,上海200433

出　　处：《国际检验医学杂志》2014年第11期1471-1473,共3页International Journal of Laboratory Medicine

摘　　要：目的：对基于量子点荧光的免疫渗滤快速定量检测血清 C 反应蛋白（CRP）方法进行初步探索，旨在建立一种较好的快速定量检测方法。方法采用双抗夹心法原理在免疫渗滤板上建立自制量子点和量子点-抗体复合物快速免疫检测法，其结果在紫外光照射下进行荧光定性检测。采用激光器和荧光光谱仪相结合的方法，可对荧光检测结果进行定量分析。结果定性检测到 CRP 的最低浓度为0．156 mg/L；定量检测 CRP 浓度范围在0．1～100．0 mg/L 的样本，其检测荧光值与浓度有线性对应关系，线性拟合方程为：log（Y ）＝0．563log（X）＋4．570，r2＝0．958。结论荧光免疫渗透快速定量法可对血清 CRP 进行定量检测；量子点免疫标记技术平台具有开发新型免疫诊断试剂的潜力。Objective To study the feasibility of using fluorescent immunofiltration test based on quantum dots （QDs）for rapid and quantitative detection of C-reactive protein.Methods Based on homemade QDs and QDs-antibody bioconjugates,an immune detection method was established via the double antibodies sandwich technique on the immunochromatography card.The test results could be read under the irradiation of UV light,and quantitative results could be measured through the combination of a laser and fluorescent spectroscopy.Results Under UV light irradiation,the minimum detection concentration of CRP was 0.156 mg/L.Using the quantitative detection method,the fluorescent intensities on the cards could be established a linear relationship with the concentration of CRP,and the linear equation was that log（Y ）=0.563 log（X）＋4.570,r^2 =0.958.Conclusion The fluorescent quantum dot immunofiltration assay can be used for quantitative detection of CRP;The quantum dots immuno-labels have the potential to develop new type of immune-diagnostic reagents.

关 键 词：量子点 荧光 免疫渗滤 C 反应蛋白 

分 类 号：R446.6[医药卫生—诊断学]