qRT-PCR检测斯氏并殖吸虫不同虫期PsMt01基因表达研究  

DETECTIONOFPSMT01INDIFFERENTSTAGEOF PARAGONIMUS SKRJABINI BY REAL-TIME FLUORESCENCE QUANTITATIVE QRT-PCR

作　　者：宋蓓[1] 康熙雄[1] 牛靖萱[2] 王英[2] 张锡林[2] 

机构地区：[1] 首都医科大学附属北京天坛医院实验诊断中心,北京100050 [2] 第三军医大学基础部病原生物学教研室,重庆400038

出　　处：《寄生虫与医学昆虫学报》2014年第1期1-5,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：重庆市自然科学基金项目（No.2008 BB5055）;四川省教育厅科研基金项目（No.2005 A067）;日本第二期健康科学基金项目（JPH20070512）

摘　　要：建立实时荧光定量qRT-PCR 方法检测斯氏并殖吸虫不同虫期免疫诊断相关的虫体蛋白 PsMt 01 mRNA的表达，探讨其生物学功能。采用Trizol方法提取虫卵、囊蚴、幼虫、童虫、成虫的总RNA，将其反转录为cDNA，以qRT-PCR方法检测PsMt 01 mRNA的变化。研究表明， PsMt 01 mRNA的表达随发育进程呈现逐步升高，在0～7周的幼虫期（42.56±0.35）和童虫期（44.12±0.56）达到峰值。因此推断PsMt 01蛋白在虫体发育的早期发挥着重要作用，可能与免疫逃避和组织迁移等活动相关。PsMt 01 mRNA biological function was forecasted by using the method of real-time fluorescence quantitative （qRT-PCR） in different stage of Paragonimus skrjabini.Total RNA was extracted with Trizol reagents from the eggs, larvae, metacercaria, immature worm schistosomulum, adult stages respectively and transcribed reversely into cDNA.qRT-PCR was used to detect the expression level of PsMt 01 mRNA.The results of qRT-PCR displayed that the expression of PsMt01 mRNA increased gradually with the development of present, especially in the period of larva-5w （42.56 ±0.35） and larva-7w （44.12 ±0.56） within weeks, PsMt01 mRNA reached the peak level.Accordingly, we concluded that PsMt 01 may play an important role during the early P.skrjabini development stage, which may be associated with immune escape and organizing migration activities.

关 键 词：斯氏并殖吸虫 qRT—PCR PsMt01 

分 类 号：S662.5[农业科学—果树学]