HPLC测定香砂枳术丸中白术内酯Ⅰ和Ⅲ的含量  

Determination of AtractylenolideⅠand Atractylenolide Ⅲ in Xiangshazhizhu Wan by HPLC

作　　者：何培根[1] 李志浩[1] 刘菁[1] 

机构地区：[1]湖北医药学院附属东风医院药学部,湖北十堰442008

出　　处：《现代仪器与医疗》2014年第2期64-66,共3页Modern Instruments & Medical Treatment

摘　　要：目的建立同时测定香砂枳术丸中白术内酯Ⅰ和Ⅲ含量方法.方法：采用DIONEXC18 色谱柱（250mm×4.6mm,5μm）,流动相：乙腈：甲醇：0.1% 磷酸溶液（11 ：46 ：43）,检测波长为222nm,柱温：35℃,流速为1.0mL·min^-1.结果：白术内酯Ⅰ和Ⅲ分别在0.1 ～ 2.0μg·mL^-1（γ=0.9997）和0.2 ～ 4.0μg · mL^-1（γ=0.9998）浓度范围内线性关系良好,平均加样回收率分别为98.7%,98.0% ;RSD 分别为0.68%、1.15%.结论：本方法简便,准确,重复性好,可用于该制剂的质量控制.Objective:To establish a method for the determination of atractylenolideⅠand atractylenolide Ⅲ in Xiangshazhizhu Wan. Method:The reversed phase HPLC system consisting the DIONEX C18(250mm×4.6mm,5μm)column,the mobile phase consisted of Acetonitrile(11)-Methanol(46)- 0.1%H3PO4(43), the UV detector was set at 222nm, the flow rate was 1.0mL·min-1,the column temperature was 35℃. Results The linear response range were0.1~ 2.0μg·mL-1(γ=0.9997)and 0.2~4.0μg·mL-1(γ=0.9998),respectively. The average recovery of atractylenolideⅠand atractylenolide Ⅲ were 98.7% and 98.0%, RSD were 0.68%,1.15% respectively. Conclusions:The assay demonstrated that the method had adequate and selectively to measure the concentrations of simple, accurate and repeatable, it could be used as a reliable tool for the determination of atractylenolideⅠand atractylenolide Ⅲ in Xiangshazhizhu Wan.

关 键 词：香砂枳术丸 白术内酯Ⅰ 白术内酯Ⅲ HPLC 

分 类 号：O657[理学—分析化学]