Preparation and Biosafety Evaluation of the Peptide Dendron Functionalized Mesoporous Silica Nanohybrid  

作　　者：Dayi Pan Chunhua Guo Kui Luo Zhongwei Gu 

机构地区：National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610064, China Email: Kui Luo ([e-mail]), Zhongwei Gu ([e-mail])[*]National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan 610064, China, Tel.: [phone] Fax: [phone]

出　　处：《Chinese Journal of Chemistry》2014年第1期27-36,共10页中国化学（英文版）

基　　金：The work was supported by National Basic Research Program of China (National 973 program,No.2011CB606206),National Natural Science Foundation of China (51133004 and 81101099) and the Excellent Young Teachers Program of Sichuan University (2012SCU04A06).

摘　　要：Mesoporous silica nanoparticles (MSNs) have been extensively studied and proposed as promising candidates for numerous biomedical applications.In this study,we report the design,preparation,characterization and biosafety evaluation of peptide dendron functionalized mesoporous silica nanohybrid.This nanohybrid was prepared by surface modification of MSNs via click reaction of azido-MSNs with alkynyl peptide dendrons,and characterized by TEM,SEM,TGA,dynamic light scattering (DLS).In vitro cytotoxicity of the nanohybrid was evaluated against different cell lines by CCK-8 assay.The in vivo toxicity evaluation was measured by body weight shift,blood routine test and histological analysis,suggesting that the peptide functionalized nanohybrid possessed good biocompatibility due to the non-observed significant side effects to normal organs of healthy mice.Overall,considering our results we believe that the peptide dendron functionalized mesoporous silica nanohybrid is very promising in further biomedical applications.Mesoporous silica nanoparticles (MSNs) have been extensively studied and proposed as promising candidates for numerous biomedical applications.In this study,we report the design,preparation,characterization and biosafety evaluation of peptide dendron functionalized mesoporous silica nanohybrid.This nanohybrid was prepared by surface modification of MSNs via click reaction of azido-MSNs with alkynyl peptide dendrons,and characterized by TEM,SEM,TGA,dynamic light scattering (DLS).In vitro cytotoxicity of the nanohybrid was evaluated against different cell lines by CCK-8 assay.The in vivo toxicity evaluation was measured by body weight shift,blood routine test and histological analysis,suggesting that the peptide functionalized nanohybrid possessed good biocompatibility due to the non-observed significant side effects to normal organs of healthy mice.Overall,considering our results we believe that the peptide dendron functionalized mesoporous silica nanohybrid is very promising in further biomedical applications.

关 键 词：mesoporous silica nanoparticles peptide dendrimer NANOHYBRID toxicity BIOSAFETY 

分 类 号：O6[理学—化学]