植物乳杆菌F的筛选及对大鼠体脂的影响  

Screening of the hypocholesterolemic effect and body fat control ability of Lactobacillus plantarum F

作　　者：辛金鸽 倪学勤[1,2] 曾东[1,2] 王鹤松[1] 李涛[1] 沈雪娇[1] 

机构地区：[1]四川农业大学动物医学院/动物微生态研究中心,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国农业大学学报》2014年第1期137-142,共6页Journal of China Agricultural University

基　　金：教育部留学回国人员科研启动基金资助项目(200835-1);四川省学术带头人培养基金项目

摘　　要：为分离具有降胆固醇功能的菌株,并研究其对大鼠脂肪沉积的影响,通过模拟胃肠道环境以及降胆固醇试验,分离得到一株植物乳杆菌F(Lactobacillus plantarum F),在培养液中的胆固醇脱除率为55.03%。用不同浓度的该菌液饲喂SD大鼠((130±2)g)8周(FⅠ组、FⅡ组和FⅢ组:分别灌胃2×106、2×108和2×1010 CFU/mL菌液)并饲喂高脂饲料。结果表明:试验Ⅰ、Ⅱ组体脂指数(腹部脂肪、肾周脂肪垫和附睾脂肪垫)、体重、肝脏体指数、甘油三酯(TG)、总胆固醇(TC)、谷丙转氨酶(ALT)和低密度脂蛋白胆固醇(LDL-C)相比高脂组均显著降低(P<0.05或P<0.01);同时,高密度脂蛋白胆固醇(HDL-C)均增加,且试验Ⅰ组变化极显著(P<0.01)。另外,试验组盲肠内乳酸菌和双歧杆菌属数量均显著升高(P<0.05或P<0.01)。结果显示,植物乳杆菌F对于高脂饲料引起的大鼠肥胖具有明显的抑制作用,并与肠道菌群的改变有一定关系。The strain F with hypocholesterolemic activity was screened by imitating the gut conditions and reducing the cholesterol level in vitro,and its ability of reducing body fat accumulation in mice was investigated.Strain F was identified as Lactobacillus plantarum and could reduce 55.03％ of cholesterol in the medium.Male SD rats with an average weight of (130 ± 2) g were administered with different concentrations of strain F at 2 × 106,2 × 108 or 2 × 1010 CFU/mL (group Ⅰ,Ⅱ and Ⅲ) for 8 weeks respectively,and were fed with high-fat feed.Compared with the high-fat group,the adipose tissue mass (abdominal fat,perirenal fat pad and epididymal fat pad),body weight,growth indexes of liver,TG,TC,ALT and LDL-C in group Ⅰ and Ⅱ significantly reduced (P＜0.05 or P＜0.01),meanwhile,HDL-C was improved in the three F groups,especially in group Ⅰ (P ＜ 0.01).In addition,the number of Lactobacillus and Bifidobacterium significantly increased in the three F groups (P＜0.05 or P＜0.01).L.plantarum F with cholesterolreducing function could reduce fat accumulation and enrich gut microbiota.

关 键 词：植物乳杆菌F 降胆固醇 脂肪沉积 抑制作用 

分 类 号：S831.2[农业科学—畜牧学] TS255.54[农业科学—畜牧兽医]