枯草芽孢杆菌OKB105产生的surfactin防治烟草花叶病毒病及其机理研究  

Effect and Mechanism of Controlling TMV Disease on Tobacco by Surfactin Produced by Bacillus subtilis OKB105

作　　者：任鹏举[1] 谢永丽[1] 张岩[1] 伍辉军[1] 高学文[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《中国生物防治学报》2014年第2期216-221,共6页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31100056);高等学校博士学科点(20100097120011);公益性行业(农业)科研专项(201303015);国家"863"计划(2012AA101504)

摘　　要：本文研究枯草芽孢杆菌产生的脂肽化合物表面活性素surfactin对烟草花叶病毒病的防治效果,并检测烟草防卫相关基因的表达情况以解析抗病机理。利用6 mol/L HCl沉淀枯草芽孢杆菌OKB105的去细胞培养液,通过甲醇抽提,Sephadex LH20层析柱获得脂肽纯化物。经MALDI-TOF-MS和血平板溶血试验检测表明,纯化物中仅含有表面活性素surfactin。将表面活性素配成0.01 g/L溶液,处理烟草并接种TMV。结果表明,surfactin对烟草花叶病毒病有显著的防治效果,病斑抑制率为70.9%。通过Real-time PCR检测烟草的防卫相关基因的表达情况结果发现,烟草SA信号通路的PR1、PR3和PR5,以及ET通路的ETR1表达量显著提高,说明surfactin诱导了烟草依赖于SA和ET信号通路的诱导系统抗性(induced systemic resistance,ISR)反应。In order to interpret resistance mechanism, controlling effect of surfactin against TMV on tobacco and the expression pattern of defense-related genes were investigated. Liquid culture of Bacillus subtilis OKB105 was precipitated by 6 mol/L HCl. The crude extract of lipopeptides was dissolved by methanol and separated by LH20 chromatographic column. The crude extract detected surfactin only by MALDI-TOF-MS and hemolytic activity analysis. Tobacco was infected with TMV after pretreated by 0.01 g/L surfactin, the result showed that surfactin could control TMV efficiently, the controlling effect reached to 70.9%. The expressions of SA-pathway genes PR1, PR3, PR5, and ET-pathway gene ETR1 were obviously increased by real-time PCR test. The results demonstrated that surfactin could elicite ISR (induced systemic resistance) reaction which depend on SA-pathway and ET-pathway.

关 键 词：SURFACTIN 烟草花叶病毒病 防治效果 诱导系统抗性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] Q936[农业科学—植物保护]