京尼平交联的丝素蛋白-壳聚糖微球的制备及表征  

Preparation and characterization of genipin-crosslinked silk fibroin/chitosan microspheres

作　　者：叶漫文[1] 曾曙光[1] 高文峰[1] 容明灯[1] 郭泽鸿[1] 石勇[1] 干辉勇 

机构地区：[1]南方医科大学附属口腔医院广东省口腔医院,广东广州510220 [2]东莞市三局医院,广东东莞523710

出　　处：《日用化学工业》2014年第6期328-332,共5页China Surfactant Detergent & Cosmetics

基　　金：广东省科技计划社会发展项目(2011B031300022);广东省卫生厅基金资助项目(B2012031)

摘　　要：以乳化交联法制备京尼平交联的丝素蛋白(SF)-壳聚糖(CS)微球,考察了SF,CS和京尼平的用量对微球的表观形态、粒径、溶胀率和物理化学性质的影响。结果表明:以京尼平1.0 g,CS和SF各0.2 g;京尼平0.05 g,CS和SF各0.2 g;京尼平0.5 g,CS 0.2 g,SF 0.4 g;京尼平0.5 g,CS 0.2 g,SF 0.1 g;京尼平0.1 g,CS 0.2 g,SF 0.1 g等5种组合制备出的微球,形态较为规则,表面较光滑,粒径分布均匀,平均粒径分别为71.13,146.60,74.09,89.00和109.83μm。经FTIR和XRD证实,京尼平使得SF和CS的氨基发生交联,SF的结构从α螺旋状或者无序蜷曲状改变为β折叠状。Genipin- crosslinked silk fibroin （SF）/chitosan （CS） microspheres was prepared by emulsion crosslinking technique. Effect of dosage of CS, SF and genipin on surface morphology, size distribution, swelling ratio and physicochemical property of the microspheres were examined. Results showed that the microspheres which prepared by 1.0 g geuipin,CS 0.2 g and SF 0.2 g;0.05 g genipin,CS 0.2 g and SF 0.2 g;0.5 g genipin,CS 0.2 g and SF 0. 4g;0.5 g genipin,CS 0.2 g and SF 0.1 g;0.1 g genipin,CS 0.2 g and SF 0.1 g, are rather regularly spherical and well distributed in shape, and their average diameters are about 71.13,146.60,74.09,89.00 and 109.83 μm, respectively. Characterization from FTIR and XRD results confirmed that genipin causes the crossliuking reaction of the amino groups of SF and CS. Further, genipin could induce structure of the SF from random coil shape or α - helix shape to β - folded sheet shape.

关 键 词：丝素蛋白 壳聚糖 微球 制备 交联 

分 类 号：O629.73[理学—有机化学] O636.1[理学—化学]