μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤的影响  

Effect of μ-opioid receptor agonist on acute lung injury induced by trauma-LPS two hits in rat model

作　　者：陈功[1,2] 陈绪贵[2] 

机构地区：[1]广东医学院 [2]北京军区总医院附属八一脑科医院麻醉科

出　　处：《临床麻醉学杂志》2014年第6期594-597,共4页Journal of Clinical Anesthesiology

摘　　要：目的探讨μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤的影响。方法 SD成年大鼠32只,随机分为正常对照组(N组)、空白对照组(B组)、DAMGO组(D组)和DAMGO+μ阿片受体拮抗剂CTOP组(DC组)。采用双侧股骨中段闭合性骨折6h后腹腔注射脂多糖(Lipopolysaccharides,LPS)5 mg/kg造模。N组只麻醉不造模,B组造模15min后腹腔注射等量生理盐水,D组注射DAMGO 200 μg/kg,DC组注射CTOP 600 μg/kg,5 min后注射DAMGO200 μg/kg。药物注射6h后行动脉血气分析,观察并比较肺组织病理改变,检测肺组织干湿重比、肿瘤坏死因子-α(TNF-α)、IL-6、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活性。结果二次打击后大鼠肺功能降低,肺组织出现了明显炎症反应。与D组比较,B、DC组动脉血PaO2、pH值明显降低;肺组织Smith评分明显升高(P<0.05);肺组织干湿重比明显降低(P<0.05);肺组织MDA、TNF-α、IL-6含量明显升高(P<0.05),SOD活性明显降低(P<0.05)。结论μ阿片受体激动剂DAMGO对创伤-内毒素二次打击所致大鼠急性肺损伤有一定保护作用。Objective To explore the effect of μ-opioid receptor agonist on acute lung injury induced by trauma-LPS two hits in rat model. Methods Thirty-two aduld SD rats, were randomized in four groups（n=8） ： normal control group（group N）, blank control group（group B）, DAMGO group （group D）and DAMGO＋CTOP group（group DC）. A rat model was made by intraperitoneal injection of LPS at 6 hours after fracturing bilateral thighbone. Group N didn＇t receive trauma and LPS, only anesthesia. The rats in group D received 200 μg/kg of DAMGO, group DC received 600 μg/kg of CTOP and 200 μg/kg of DAMGO , group B obtained the same amount of saline. 6 hours after treatment, the arterial blood was collected for blood gas analysis, the lungs was harvested to observe lung tissue pathology change and dry-weight/wet-weight ratio, and the levels of MDA, TNF-α, IL-6, SOD activities in lung tissue were determined. Results The results of pathological observation showed that there was obvious inflammatory reaction in lung tissues after two-hits. Compared with group D, PaO2, pH and dry-weight/wet-weight ratio were significantly lower in group B and group DC（P〈 0.05）, The score of Smith were significantly increased（P〈0.05）. The levels of MDA,TNF-α, IL-6 in lung tissue were significantly reduced in group D than those in group B and group DC（P〈0.05）, SOD activities in group D were significantly higher than those in group B and group DC（P〈0. 05）. Conclusion μ-Opioid receptor DAMGO agonist has protective effect on acute lung injury induced by trauma-LPS two hits in rat model.

关 键 词：μ阿片受体DAMGO CTOP 急性肺损伤 二次打击 

分 类 号：R563.8[医药卫生—呼吸系统]