右美托咪定对大鼠肢体缺血-再灌注所致急性肺损伤的影响  

Effects of dexmedetomidine on acute lung injury induced by hind limb ischemia-reperfusion in rats

作　　者：郝楠楠[1] 雷志礼[2] 陈晖[2] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室,221004 [2]武警总医院麻醉科

出　　处：《临床麻醉学杂志》2014年第6期602-605,共4页Journal of Clinical Anesthesiology

摘　　要：目的探讨右美托咪定对大鼠肢体缺血-再灌注所致急性肺损伤的影响及相关机制。方法雄性清洁级SD大鼠120只,随机分为三组:对照组、缺血-再灌注组、右美托咪定组。在术前、肢体缺血-再灌注后0、1、3、6h测定大鼠PaO2、肺组织湿干比重(W/D)、血清SOD活性、MDA浓度及TNF-α水平,并观察肺组织病理学变化。结果与对照组比较,缺血-再灌注组再灌注后0、1、3、6h肺组织W/D和TNF-α水平明显升高(P<0.05),PaO2明显降低(P<0.05);再灌注1、3、6 h MDA浓度明显升高(P<0.05),SOD活性明显降低(P<0.05)。与缺血-再灌注组比较,右美托咪定组再灌注后6h肺组织W/D和TNF-α明显下降、PaO2水平明显升高(P<0.05),再灌注后3、6 h SOD活性明显升高(P<0.05),再灌注后1、3、6hMDA浓度明显降低(P<0.05)。结论右美托咪定可以减轻大鼠肢体缺血-再灌注所致的急性肺损伤,其机制与减轻炎性反应和抗氧化应激有关。Objective To explore whether dexmedetomidine（Dex） could mitigate acute lung injury induced by hind limb ischemia reperfusion（I/R）. Methods One hundred and twenty SD rat weighting 250-300 g were allocated to receive hind limb I/R, I/R plus Dex （25 μg/kg Dex was intraperitoneal injection 30 rain before iscbemia） and norm control group, and each group was further divided into five subgroups： before operation and 4 h ischemia followed by 0,1,3 and 6 h reperfusion. After euthanization, lung W/D weight ratio, PaO2 , SOD, MDA and TNF-α were determined. Results Compared with group C, hind limb I/R injury significantly increased serum TNF-α concentration and W/D ratio, with significantly decreasing PaO2 level at any time of reperfusion in the group I/R, the concentration of MDA increased and the SOD activity decresed at 1,3,6 h of reperfusion. Conversely, W/D ratio as well as the concentration of TNF-α in the serum of the UR plus group Dex were significantly lower than those of the I/R at 6 h of reperfusion, PaO2 level increased respectively; the concentration of MDA decresed at 1,3,6 h of reperfusion and the SOD activity increased at 3,6 h of reperfusion in UR plus group Dex. Conclusion Dex mitigates acute lung injury induced by unilateral hind limb I/R in rats. The mechanisms may involve attenuating oxidative stress and inhibiting inflammatory response.

关 键 词：右美托咪定 急性肺损伤 缺血-再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]