急性膀胱过度充盈对逼尿肌功能和超微结构的影响  

The changes of function and ultrastructure of detrusor cell following acute urinary bladder overdis-tension

作　　者：马勇[1] 周林[1] 郭剑明[1] 王杭[1] 王国民[1] 

机构地区：[1]复旦大学附属中山医院泌尿外科,上海200032

出　　处：《中华实验外科杂志》2014年第6期1172-1174,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年科学基金资助项目（30700831）;上海市卫生局青年科研项目（2006Y016）

摘　　要：目的观察膀胱腔内短期高压力状态对逼尿肌细胞形态及功能的影响。方法将雄性新西兰白兔14只分为2组，每组7只。实验组建立膀胱过度充盈动物模型，保持3h；对照组仅插入导尿管，不施行膀胱过度充盈灌注。所有实验兔均饲养3d后解剖。分别测量膀胱重量并检测逼尿肌功能，对膀胱逼尿肌细胞超微结构进行观察。结果实验组膀胱重量为（3．45±0．36）g，对照组膀胱重量为（4．02±0．47）g，两组比较差异无统计学意义（P〉0．05）；实验组在三磷酸腺苷（ATP）溶液、阿托品溶液和无钙液中最大张力较对照组明显增加（P〈0．05）；实验组在阿托品溶液、无钙液、高K^＋液中最大张力产生速度较对照组明显增加（P〈0．05）。对照组平均每个视野中内质网面积为（0．203±0．097）μm^2，实验组为（1．748±0．772）μm^2，实验组明显大于对照组（P〈0．05）；对照组线粒体平均相对密度为1．681±0．219，实验组为1．142±0．085，实验组明显小于对照组（P〈0．05）。结论急性膀胱过度充盈可以导致逼尿肌收缩能力增强，这一变化有其超微结构基础。Objective To evaluate the contractive function and ultrastructure changes of detrusor following acute bladder overdistension. Methods 14 New Zealand white male rabbits were divided into two group, 7 rabbits in each. Overdistension group was induced for 3 hours by forced diuresis and ligation of urethra to volumes 130% of maximum intravesical volume, control group was catheterized with an 8 F catheter but not instillation. The weight of each bladder and function and uhrastructure of detrusor were examined after 3 d. Results The weight of bladder is （ 3.45 ± 0. 36） g in overdistension group and （4. 02 ± 0. 47 ） g in control group. The contractive strength of per 100 mg detrusor of overdistension group increased significantly in adenosine-triphosphate （ATP） solution, atropine solution and Ca^2＋ free solution; The contractive velocity increased significantly, in atropine solution, Ca^2＋ free solution and high K^ ＋ solusion; The average area of rough endoplactmic reticulum in overdistension group was （ 1. 748 ± 0. 772 ） μm^2 in each unit cytoplasm area versus （ 0. 203 ± 0. 097 ） μm^2 in control group （ P 〈 0. 05 ）. The relative density of mitochondrion was 1. 142 ± 0. 085 in overdistension group versus 1. 681 ± 0. 219 in control group （P 〈 0. 05 ）. Conclusion Overdisteusion of bladder will increase the constructive function of detrusor, and there are structure foundations of the changes.

关 键 词：膀胱 过度充盈 内质网 线粒体 

分 类 号：R694[医药卫生—泌尿科学]