基于调控大鼠结肠c-kit mRNA表达的中药益智仁燥性及盐炙润燥研究  

Study of Fructus Alpiniae Oxyphyllae Dry Effects and Salt-Processed Moistening-Dryness by Regulating the Expression of Rats' Colon c-kit mRNA

作　　者：王瑾[1] 谭茂兰[1] 汪云伟[1] 黄勤挽[1] 钟振东[2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]四川省人民医院实验动物研究所,四川成都610212

出　　处：《成都中医药大学学报》2014年第2期1-3,9,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:81001639);教育部博士点新教师基金(编号:2010513120002)

摘　　要：目的:观察益智仁燥性及盐炙后对正常大鼠结肠c-kit mRNA表达的影响,从而在肠道传输能力方面研究益智仁盐炙润燥作用及其关键环节。方法:将SPF级SD大鼠分为正常动物组、生益智仁组、盐益智仁组、清水炒益智仁组、生益智仁加盐组、生益智仁加增液汤组6组,各组动物灌胃给药1次/d,连续28 d,在第28 d处死动物剖取结肠组织,采用实时荧光定量聚合酶链式反应对结肠中c-kit mRNA的表达进行检测。结果:与正常动物组比较,生益智仁组结肠c-kit mRNA表达显著降低(P<0.01);与生益智组比较,盐益智仁组、清水炒益智仁组、生益智仁加盐组、生益智仁加增液汤组的结肠c-kit mRNA表达均显著增加(P<0.05或P<0.01)。结论:益智仁生品能降低正常大鼠结肠中c-kit mRNA的表达,进而影响肠道Cajal间质细胞造成便秘体现其燥性效应;而益智仁盐炙后能降低益智仁引起的肠道燥性效应,具有润燥作用,其作用环节与盐炙过程的加热炒制和辅料食盐均有关。Objective： To study the effect on the expression of c-kit mRNA of normal rats colon of Fructus Alpiniae Oxyphyllae （FAO） dry effects and after the salt processing, so as to study the mechanism of salt-processed moistening-dryness and its key links of FAO in terms of intestinal transmission ability. Methods： The SPF SD rats were divided into 6 groups： normal group, crude FAO group, FAO stir-frying with salt water and water groups, crude FAO coupled with salt group, FAO and increased liquid soup combi- nation group. Each group was orally administered once a day for 28 d. All rats were sacrificed on the 28th day, and dissected the colon, then detected the expression of c-kit mRNA by real-time fluorescent quantitative PCR. Results： Compared with normal group, the ex- pression of c-kit mRNA of crude FAO group was decreased significantly （P 〈0. 01 ） ; in comparison with crude FAO group, the ex- pressions of c-kit mRNA of FAO stir-frying with salt water and water groups, crude FAO coupled with salt group, FAO and increased liquid soup combination group were increased significantly （P 〈0. 05 or P 〈 0. 01 ） . Conclusion： Crude FAO can lower the expres- sion of c-kit mRNA of normal rats colon, to influence intestinal cells of Cajal, then lead to constipation and reflect its dry effects; However, FAO stir-frying with salt water can lower intestinal dry effects which caused by FAO, and had the effect of moistening-dry- ness, the mechanism was related to stir-frying and salt in the process of stir-frying with salt water.

关 键 词：益智仁 盐炙 大鼠结肠 C-KIT mRNA 

分 类 号：R961.1[医药卫生—药理学] R283.1[医药卫生—药学]