基于δ^(15)N稳定同位素分析的人工防护林大型土壤动物营养级研究  

Study on the trophic levels of soil macrofauna in artificial protection forests by means of stable nitrogen isotopes

作　　者：张淑花[1,2] 张雪萍[1] 

机构地区：[1]哈尔滨师范大学地理环境遥感监测重点实验室,哈尔滨150025 [2]绥化学院农业与水利工程学院,绥化152061

出　　处：《生态学报》2014年第11期2892-2899,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(41371072);黑龙江省教育厅科学技术资助项目(12521655)

摘　　要：稳定同位素δ15N是分析生态系统中的营养关系的重要方法。本研究采用该方法分析了人工防护林土壤动物的营养级及它们之间的营养关系。对3个地区人工杨树林12类大型土壤动物及其土壤、细根及植物枯落叶的稳定同位素δ15N分析结果显示,不同环境条件下土壤动物δ15N具有显著差异性(F=38.067,P<0.001),土壤动物δ15N值与土壤、植物细根及枯落叶δ15N值之间有明显的相关性,与全氮含量没有明显的相关性;根据不同土壤动物δ15N值的状况,12种土壤动物在土壤生态系统中大都处于第二至第四营养级,处于较低营养级位置的动物主要有大蚊幼虫(Tipulidae larvae)、蚯蚓(Lumbricidae)、金龟甲幼虫(Scarabaeidae larvae)等类群,较高营养级的为蜈蚣(Scolopendromorpha)、隐翅甲成虫(Staphylinidae adult)、叩甲成虫(Elateridae adult)、线蚓(Enchytraeidae)等动物,同类土壤动物在不同样地中营养级位置存在一定的差异。Stable isotope technology is a very effective method for ecological research, and the stable nitrogen （N） isotope （δ^15） is widely used to investigate food chains and food webs in ecosystems. In this study, δ^15 was used to analyze the trophic level of soil macrofauna in three artificial protection forests. The forests selected for this study belonged to three North Shelterbelts located in the Daowai and Acheng districts of Harbin, Heilongjiang Province. The total N and δ^15 of 12 soil macrofauna samples were investigated in the three sample forests in September 2010 and the corresponding values in soil, leaf litter and fine plant roots were also determined. The results showed that the δ^15 values of the soil macrofauna in each sample spanned a large range; between 2‰ and 6.40‰ in Wanbao, Daowai district, between -0.50‰ and 4.10‰ in Yangshu, Acheng district, and between 4.20‰ and 9.96‰ in Pingshan, Acheng district. The average values of δ^15 in the three samples varied greatly; 5.34‰, 1.88‰ and 8.45‰ in WanBao, Yangshu and Pingshan, respectively. Statistical analysis showed that the δ^15 of soil macrofauna under different conditions was significantly different （F=38.067, P〈0.001） while the total N values of the macrofauna, soil, fine roots and leaf litter had no obvious difference （F=0.190, P=0.828） in the three plots. Correlation analysis showed a significant positive correlation of the total N values in the three samples. This indicates that the environmental differences had no influence on the total N content of the soil macrofauna. The correlation analysis showed that the δ^15 values of the soil macrofauna had an obvious correlation with the δ^15 values of the soil, fine roots and leaf litter; the correlation coefficients were all 〉0.6 （P 〈 0.05 or P 〈 0.01）. However, the δ^15 values had no correlation with the total N content or their correlation coefficients[（r=0.571 （P=0.026）, 0.295 （P=0.285） and 0.404 （P=0.135） in Wanbao, Pingshan and Ya

关 键 词：稳定同位素 δ^15N 土壤动物 营养级 

分 类 号：S714.3[农业科学—林学]