外源水杨酸对毒死蜱胁迫下蔬菜上海青抗氧化防御反应的影响  

Effects of exogenous salicylic acid on anti-oxidant defensive responses of Brassica chinensis L.exposed to chlorpyrifos

作　　者：刘向阳[1] 李英涛[1] 高飞[1] 赵特[1] 周琳[1] 

机构地区：[1]河南农业大学植物保护学院/河南省新型农药创制与应用重点实验室,郑州450002

出　　处：《华中农业大学学报》2014年第4期66-71,共6页Journal of Huazhong Agricultural University

基　　金：河南省重点科技攻关项目(042102350033)

摘　　要：在室内栽培条件下，观察毒死蜱（chlorpyrifos，CPF）对蔬菜上海青造成的胁迫和水杨酸（salicylicacid，SA）对上海青应对毒死蜱胁迫的影响。测定结果显示：喷施91．2、182．4、273．6mg／L的毒死蜱后，短期内（2～4d）上海青中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性受到抑制，而过氧化物酶（POD）活性则有所升高，且伴随POD活性升高的还有丙二醛（MDA）、脯氨酸（Pro）和谷胱甘肽（GSH）含量的增加，表明毒死蜱在喷施后短期内对上海青具有一定的胁迫；高浓度外源水杨酸可缓解毒死蜱对上海青SOD和CAT的直接抑制，使SOD和CAT活性增强，但对POD活性和MDA、Pro含量无明显影响，表明在供试浓度范围内，外源水杨酸可部分缓解毒死蜱对上海青的胁迫。The effects of chlorpyrifos and exogenous salicylic acid on anti-oxidant defensive response of Brassica chinensis were investigated by indoor cultivation experiments. The activities of superoxide dismutase （SOD） and catalase （CAT） in B. chinensis were suppressed significantly, whereas the activity of peroxidase （POD） was increased significantly within 4 days after being sprayed with chlorpyrifos. In addition, the contents of malondialdehyde （MDA）, proline and glutathione （GSH） significantly in- creased in a short period （2-4 day） after the spraying. These results indicated that the usage of chlor- pyrifos caused stress on B. chinensis seedlings. The inhibitions of chlorpyrifos on activities of SOD, CAT and GSH could be relieved by high concentrations of exogenous salicylic acid, whereas exogenous salicylic acid almost had no influence on the activity of POD and the contents of MDA and proline. These results suggested that exogenous salicylic acid only relieved stress on B. chinensis to some extent.

关 键 词：上海青 毒死蜱 农药胁迫 水杨酸 防御反应 

分 类 号：S436.34[农业科学—农业昆虫与害虫防治] S634.3[农业科学—植物保护]