北部湾三角棘原甲藻(甲藻门原甲藻目)的形态观察和18S rDNA序列分析  

Morphological and phylogenetic analysis of Prorocentrum triestinum isolated from the Beibu Gulf

作　　者：龙超[1] 陈宪云[1] 陈波[1] 何碧娟[1] 高程海[1] 王一兵 

机构地区：[1]广西科学院广西近海海洋环境科学重点实验室,广西南宁530007

出　　处：《热带海洋学报》2014年第2期66-71,共6页Journal of Tropical Oceanography

基　　金：广西科学院基本科研业务费资助项目(12YJ25HY10);广西自然科学基金项目(2012GXNSFEA053001;2011GXNSFE018002)

摘　　要：利用光学显微镜、分子生物学方法,对分离自广西北部湾海域的三角棘原甲藻 Prorocentrum triestinum BBW-02藻株的形态特征进行了描述,并探讨了其分子系统进化关系。三角棘原甲藻 BBW-02细胞呈长卵形或披针形,细胞长20~26μm,宽10~14μm,顶刺形如矛尖,刺丝胞孔数量较少,排列不规则,主要分布于壳板边缘,距离细胞底端1/3处壳板两侧各有2个近于对称排列的刺丝胞孔。18S rDNA序列同源检索和系统进化分析表明,三角棘原甲藻BBW-02与其他地理株系的三角棘原甲藻聚为一支,亲缘关系密切,遗传差异仅为0.000~0.001。与其他种类相比,三角棘原甲藻BBW-02与同属中的P. gracile亲缘关系最近。Prorocentrum triestinum BBW-02 strain, isolated from the Beibu Gulf of Guangxi province, was described on the basis of morphological characteristics and phylogenetic evolution relationship using light microscopy and molecular sequencing. The cells are lanceolate or heart-shaped, 20-26 μm long and 10-14 μm wide. The anterior spine is spearhead-shaped. The thichocysts of the surface are few, and mainly distribute around the margin irregularly. Two thichocysts distribute approximate symmetrically in the two-third of the theca. Homologous retrieval and system evolution analysis showed that P. triestinum BBW-02 was closely related to the other analysed P. triestinum. Compared with other species, P. triestinum BBW-02 had the most intimate relationship with P. gracile.

关 键 词：三角棘原甲藻Prorocentrumtriestinum 形态特征 分子系统进化 北部湾 

分 类 号：P735[天文地球—海洋生物学]