替米沙坦对新疆哈萨克族高血压患者外周血T细胞活化增殖后电压门控钾通道的阻断作用  

Effects of telmisartan on the voltage-gated potassium channel following T cells activation and proliferation in peripheral blood of patients with hypertension in Xinjiang Kazakh

作　　者：张邱兵 程路峰[2] 张源明[1] 袁庆艳 章桂铭 苟芳[3] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,新疆乌鲁木齐830054 [2]新疆医科大学基础医学院药理教研室 [3]泸州医学院附属医院内分泌科

出　　处：《中华高血压杂志》2014年第5期457-462,共6页Chinese Journal of Hypertension

基　　金：国家自然科学基金(81160039)

摘　　要：目的探讨替米沙坦对新疆哈萨克族高血压患者外周血T细胞活化增殖后电压门控Kv1.3钾通道的阻断作用。方法 2012年8至12月在新疆医科大学第一附属医院高血压门诊随机入选未经降压药物治疗的新疆哈萨克族高血压患者30例,男女各15例。分离其外周血T细胞进行体外培养增殖。将培养增殖后T细胞不作任何处理(空白对照组)或将替米沙坦和4-氨基吡啶(Kv1.3钾通道阻滞剂)溶解后分别加入培养液中(替米沙坦组和4-氨基吡啶组),继续培养。采用膜片钳技术记录上述3组T细胞培养0、24、48h后的Kv1.3钾通道电生理特性。结果替米沙坦组干预24和48h的Kv1.3钾通道峰电流、峰值电流密度较同一时间点空白对照组降低[24h:(866±137)比(1603±379)pA,(236±32)比(443±60)pA/pF;48h:(595±149)比(1632±375)pA,(161±30)比(417±62)pA/pF,均P<0.01]。替米沙坦对T细胞Kv1.3钾通道干预24h、48h的阻断率分别为46.7%、61.4%。4-氨基吡啶组各个干预时间点的Kv1.3钾通道峰电流、峰值电流密度较空白对照组降低[0h:(443±105)比(1581±395)pA,(117±19)比(416±126)pA/pF;24h:(261±54)比(1603±379)pA,(70±12)比(443±60)pA/pF;48h:(209±55)比(1632±375)pA,(57±18)比(417±62)pA/pF,均P<0.01]。结论替米沙坦呈时间依赖性阻断新疆哈萨克族高血压患者T细胞Kv1.3钾通道。Objective To investigate the effects of telmisartan on the voltage-gated potassium channel Kv1. 3 after T cells activation and proliferation in peripheral blood of Xinjiang Kazakh hypertensives. Methods In the Hyperten- sion Department, First Affiliated Hospital of Xinjiang Medical University, from August to December 2012, 30 Xinjiang Kazakh hypertensive outpatients （ 15 males and 15 females respectively） without antihypertensive drug ther- apy were randomly enrolled. Their peripheral blood T cells were isolated and cultured in vitro without any treat- ment（ blank control group）, or telmisartan and 4-aminopyridine （Kv1. 3 potassium channel blockers）were dissolved and put into the nutrient solution respectively（ the telmisartan group and 4-aminopyridine group）. The Kv1. 3 chan- nel electrophysiological characteristics of T cells following telmisartan or 4-aminopyridine exposure for 0, 24, 48 h were analyzed with patch-clamp techniques. Results The peak current and peak current density of Kv1. 3 channel treated with telmisartan for 24 h or 48 h were significantly inhibited as compared with the corresponding blank con- trol group [for peak current： （866±137） vs （1603±379）pA at 24 h and （595±149） vs （1632±375）pA at 48 h; for peak current density： （236±32） vs （443±60）pA/pF at 24 h and （161±30） vs （417±62）pA/pF at 48 h; all P〈 0.01]. The blocking rates after intervention with telmisartan for 24 h and 48 h were 46.7% and 61.4%, respectively. Similarly, the Kv1. 3 channel peak current and peak current density following 4-aminopyridine exposurefor various time were lower than the control group [for peak current： （443±105） vs （1581±395） at 0 h, （261±54） vs （1603±379） at 24 h and （209±55（ vs （1632±375） pA at 48 h; for peak current density： （117±19） vs （416± 126） at0 h, （70±12） vs （443±60） at 24 h and （57±18） vs （417±62） pA/pF at 48 h; all P〈0.01]. Conclusions Telmisartan displayed a time-dependent

关 键 词：高血压 T淋巴细胞 KV1 3钾通道 替米沙坦 

分 类 号：R544.1[医药卫生—心血管疾病]