胶原生物膜支架材料促进膀胱再生的组织学初步研究  

EXPERIMENTAL ASSESSMENT OF BLADDER REGENERATION BY COLLAGEN MEMBRANE SCAFFOLDS

作　　者：马超[1] 陈伟[1] 贾维胜[1] 郭宇[1] 叶钢[1] 

机构地区：[1]第三军医大学新桥医院泌尿外一科,重庆400037

出　　处：《中国修复重建外科杂志》2014年第6期773-776,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国学自然科学基金资助项目(31000442)~~

摘　　要：目的观察胶原生物膜重建膀胱后促进膀胱再生的作用，初步探索新的膀胱重建材料。方法取12只健康成年雄性SD大鼠，体重300～350g，随机分为胶原生物膜组（实验组）及假手术组（对照组），每组6只。实验组行上半膀胱切除术并胶原生物膜重建，对照组仅对膀胱作同等量翻动。术后观察大鼠一般情况，并于30d取材，行HE、Masson染色，观察膀胱再生及胶原生物膜吸收情况；行α平滑肌肌动蛋白（α-smoothmuscleactin，α-SMA）、血管假性血友病因子（vonWillebrandfactor，vWF）免疫组织化学染色，计算α-SMA染色阳性面积比及血管数。结果两组大鼠均存活至实验完成，实验组大鼠无尿漏、感染发生。组织学观察示，对照组均呈正常膀胱结构；实验组胶原生物膜黏膜面已基本被尿路上皮细胞覆盖，肌层可见少量平滑肌细胞，并见散在未降解胶原纤维。免疫组织化学染色观察，实验组可见肌纤维沿胶原生物膜长入，膜内有新生血管；实验组α-SMA染色阳性面积百分比为6．49％±2．14％，显著低于对照组的52．42％±1．78％（t=40．40，P=-0．00）；实验组血管数为（4．83±0．75）个，显著低于对照组的（14．83±1．17）个（t=17．62，P=0．00）。结论胶原生物膜可作为膀胱重建的支架材料。Objective To observe the bladder regeneration by collagen membrane scaffolds for bladder construction to find a new alternative scaffold material. Methods Twelve healthy adult male Sprague Dawley rats, weighing 300- 350 g, were randomly divided into collagen membrane scaffold group （experimental group, n=6）, and sham operated group （control group, n=6）. Upper hemicystectomy was performed and collagen scaffold was used for reconstruction in experimental group, while the bladder was turned over without bladder resection in control group. At 30 days after operation, the animals were sacrificed and grafts were harvested; HE staining and Masson staining were used to evaluate the bladder regeneration, immunohistochemical staining was performed with α-smooth muscleactin （α-SMA） and yon Willebrand factor （vWF） markers to evaluate the percentage of α-SMA positive area and capillary number. Results The rats of 2 groups survived to the end of the experiment, and no urine leakage or infection was observed in experimental group. Histologically, control group presented a pattern of normal bladder structure, experimental group presented a pattern of almost normal urothelium with a small amount of smooth muscle cells and a thin layer of undegraded collagen fibers. Immunohistochemically, experimental group showed ingrowth of smooth muscle fibers and new capillary formation along the collagen membrane scaffolds. The percentage of α-SMA positive area and capillary number in experimental group were significantly lower than those in control group （6.49% ±2.14% vs. 52.42% ± 1.78% and 4.83 ±0.75 vs. 14.83 ± 1.17, respectively）（t=40.40, P=0.00; t=17.62, P=0.00）. Conclusion The collagen membrane scaffolds could be an effective scaffold material for bladder reconstruction.

关 键 词：膀胱组织工程 胶原生物膜 支架材料 膀胱重建 

分 类 号：R318.08[医药卫生—生物医学工程]