^(15)N示踪法测定海洋反硝化速率中^(29)N_2浓度的准确定量  

Accurately quantification of ^(29)N_2 concentration in ^(15)N tracer derived marine denitrificaiton rates

作　　者：曾健[1] 陈敏[1,2] 郑敏芳[1] 邱雨生[1,2] 

机构地区：[1]厦门大学海洋与地球学院,福建厦门361005 [2]近海海洋环境科学国家重点实验室,福建厦门361005

出　　处：《海洋学报》2014年第6期10-17,共8页

基　　金：国家自然科学基金杰出青年基金项目(41125020);国际海域资源调查与开发"十二五"课题(DY125-13-E-01);科技基础性工作专项(2008FY110100)

摘　　要：利用15 N示踪法实测南海水体反硝化速率的研究发现,培养水样在长时间密闭放置过程中也会受到外界空气的污染,且其29N2/28N2比值恒定为0.007 35。根据空气背景中29N2/28N2比值恒定的特征,提出基于质量平衡关系校正空气N2污染的方法,通过将样品实测29N2浓度扣除由外界空气贡献的29N2浓度,可获得由生物反硝化作用所产生的29N2准确浓度,进而可计算出准确的反硝化速率。经空气29N2背景校正后,29N2浓度的偏差明显小于未经校正的结果,且29N2浓度与培养时间之间的线性相关性显著加强,凸显出空气29N2背景校正是获取准确反硝化速率的关键。鉴于15 N示踪法已被广泛应用于海洋水体与沉积物反硝化速率的测定中,所提出的空气29N2背景校正方法具有重要的意义。^15N tracer technique was widely applicated in measuring water column denitrification rate in water sampies,however,the sealed samples could be contaminated by air during a long time storage. Our study suggested that N2 in air has a constant ^29N2/28N2 ratio of 0. 007 35. According to this feature, a mass-balance calculation was proposed to calibrate the interference from air background. It helps obtain more accurately ^29N2 concentrations pro- duced by biological activity and further the denitrification rates. After the calibration, the deviations of ^29N2 concentrations were much less than those of the raw data, and the linear correlation between ^29N2 concentration and the incubation time was more significant. It is important to calibrate extra ^29N2 signals from air contamination for accurately quantification of denitrification rate, considering the ^15N technique has been widely used in pelagic and beneath denitrification rate measurements.

关 键 词：^15N示踪 反硝化速率 空气背景 ^29N2校正 

分 类 号：P734.21[天文地球—海洋化学]