抑制Bmi-1基因表达对鼻咽癌细胞生物学行为的影响  

Effects of Bmi-1 gene suppression on nasopharyngeal carcinoma cell biology behavior

作　　者：李海玉[1] 陈兴凤 余思颖 刘革力[1] 宋方洲[1] 

机构地区：[1]重庆医科大学分子医学与肿瘤研究中心,生物化学与分子生物学教研室,重庆400016

出　　处：《中国药理学通报》2014年第6期833-837,共5页Chinese Pharmacological Bulletin

基　　金：教育部高等学校博士学科点专项科研基金(No 20125503110012);教育部高等学校博士学科点新教师基金项目(No 20115503120007);重庆市自然科学基金项目(No cstc2011jjA10035)

摘　　要：目的利用小干扰RNA技术沉默Bmi-1基因的表达,探讨其对人鼻咽癌CNE-1生物学行为的影响。方法设计并化学合成了针对Bmi-1的小干扰RNA,转染具有高转移性的CNE-1细胞,利用qRT-PCR和Western blot分别检测Bmi-1基因mRNA和蛋白的表达水平。体外通过Transwell小室、Matrigel胶模型﹑流式细胞术,观察Bmi-1被沉默后对鼻咽癌细胞株CNE-1生物学行为的影响。结果 qRT-PCR和Western结果显示,Bmi-1-siRNA转染组Bmi-1基因的表达水平被有效抑制。与对照组相比,Bmi-1-siRNA转染组的细胞侵袭迁移力明显下降,细胞被阻滞在S期。结论抑制Bmi-1基因的表达可有效降低CNE-1细胞侵袭迁移的能力。Aim To investigate the effect of small inter-ference RNA-mediated silcencing of the Bmi-1 gene on cell invasion and metastasis in human nasopharyngeal carcinoma cell line CNE-1 . Methods Chemically syn-thesized siRNA targeting the Bmi-1 gene was transfect-ed into CNE-1 cells, which had high invasive and metastatic potential. The expression of Bmi-1 mRNA and protein were detected by quantative Real-time PCR and Western blot, respectively. The effects of Bmi-1 knockdown on CNE-1 cells migration and invasion were analysied by Transwell migration assay and Matrigel in-vasion assay. Results Transfected with Bmi-1 siRNA significantly down-regulated the expression of Bmi-1 mRNA and protein as compared with the control group. CNE-1 cells transfected with Bmi-1 siRNA had lower levels of invasion and migration capacity than cells in the control group. Conclusion SiRNA-media-ted silencing of the Bmi-1 gene could significantly inhibit cell migration and invasion in human nasopharyn-geal carcinoma cell line CNE-1 .

关 键 词：BMI-1 RNA干扰 侵袭 迁移 周期 增殖 凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]