白藜芦醇对K562白血病细胞抑制增殖和诱导分化的作用  

Effect of resveratrol on proliferation and differentiation in K562 cells

作　　者：赵燕娜[1] 高瑞兰[1] 汪丽佩[2] 余潇苓[1] 尹利明[1] 

机构地区：[1]浙江中医药大学附属第一医院血液病研究所,浙江杭州310006 [2]浙江中医药大学基础医学院病理学教研室,浙江杭州310053

出　　处：《中国药理学通报》2014年第6期853-856,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81373876);浙江省自然科学基金资助项目(No LY14H290004;LQ14H290003);浙江中医药大学基金资助项目(No 2013ZY10)

摘　　要：目的探索白黎芦醇对K562白血病细胞株的抑制增殖和诱导分化作用。方法不同浓度的白黎芦醇处理K562白血病细胞6 d后,利用半固体集落生成实验检测K562白血病细胞集落数;通过细胞免疫化学法和Western-blot法检测分化相关转录因子GATA-1和PU.1蛋白表达情况;流式细胞术检测分化相关抗原CD11b、CD14和CD42b阳性细胞的表达率。结果白黎芦醇能够降低K562白血病细胞集落数,且呈剂量依赖性(P<0.05);提高分化相关转录因子GATA-1和PU.1蛋白表达水平,升高K562细胞分化相关抗原CD11b、CD14、CD42b的表达阳性的细胞率,呈剂量依赖性(P<0.05)。结论白黎芦醇可能通过上调GATA-1和PU.1蛋白表达和升高CD11b、CD14和CD42b表达阳性的细胞率,诱导K562细胞向红系、粒系和巨核系方向分化,并抑制其增殖。Aim To investigate the effect of resveratrol on proliferation and differentiation in K562 cells. Methods K562 cells were treated with different con-centrations of resveratrol for 6d. The colony number of K562 cells was detected with semi-solid culture assay. Expression of GATA-1 and PU. 1 in K562 cells was respectively measured with immunocytochemistry and Western blot. Expression of differentiation related anti-gen, CD11b, CD14 and CD42b, was measured with flowcytometry on K562 cells. Results Resveratrol could significantly decrease the colony number of K562 cells in a dose-dependent manner, and enhance the ex-pression of GATA-1,PU. 1,CD11b, CD14 and CD42b in K562 cells. Conclusion Resveratrol could inhibit the proliferation and induce differentiation of K562 cells via up-regulating the expression of GATA-1 and PU. 1 protein.

关 键 词：白藜芦醇 白血病 分化 CD11B CD14 CD42B GATA-1 PU 1 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]