二母颗粒含药血清对肺腺癌A549及肝癌SMMC-7721细胞株凋亡的影响  

Effect of serum containing Ermu particles on apoptosis of human lung adenocarcinoma A549 and hepatocellular carcinoma SMMC-7721 cells

作　　者：代勇[1] 徐世军[2] 刘蓉[2] 熊敏[2] 苟玲[2] 

机构地区：[1]成都中医药大学基础医学院,成都611137 [2]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中药药理与临床》2014年第1期5-8,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家十一五支撑计划重大项目(2007BAI40B01);四川省科技支撑计划(2009SZ0052);成都市十二五科技支撑项目(11GGYB288SW-289)

摘　　要：目的:观察二母颗粒含药血清对肺腺癌A549及肝癌SMMC-7721细胞株增殖和凋亡的影响。方法:采用形态学及MTT法观察二母颗粒组(10g/kg)5%、10%、20%含药血清对A549及SMMC-7721细胞株增殖的抑制作用;通过流式细胞法观察药物对细胞凋亡的影响。结果:形态学观察表明二母颗粒含药血清使A549及SMMC-7721细胞株发生形态改变;MTT结果显示二母颗粒含药血清对A549及SMMC-7721细胞株增殖有抑制作用,且随含药血清浓度的增高以及作用时间的延长,抑制作用亦有所加强。其中20%含药血清作用于A549及SMMC-7721细胞株48h,10%含药血清作用于A549 48h,其OD值与对照组比较均有统计学差异;流式细胞技术检测结果表明20%含药血清作用于A549细胞株24h及48h,10%含药血清作用于A549 48h,与同时间段阴性对照组比较凋亡率明显升高;20%、10%含药血清作用于SMMC-7721细胞株24h及48h,对肿瘤细胞的凋亡率也有明显升高的作用。结论:二母颗粒含药血清对A549及SMMC-7721细胞株的增殖有抑制作用,其作用机制与其诱导细胞凋亡有密切的关系。Objective： To observe the effect of serum containing Ermu particles on apoptosis of A549 and SMMC-7721 cells and explore the corresponding mechanism. Method： The antiprolifemtive effect of A.549 and SMMC-7721 cells were observed with morphological examination and MTT assay. The cell apoptosis detection was carried out by using flow cytometry. Result： Morphology of A549 and SMMC-7721 cells was changed after them were exposed to different doses of drug-centained serum （5% ,10% and 20% ） for 24 or 48 h. ;Serum Containing Ermu particles inhibited the growth of A549 and SMMC-7721 ceils significantly and the growth suppression rate presented time and dose dependent relationship. The OD value of A.549 and SMMC-7721 cells were significantly decreased than control groups when them were exposed to drug-contained serum（20% ） after 48h（ P 〈 0.05 ）. The OD value is also decreased when A549 ceils exposed to drug-contained serum（ 10% ） for 48h （ P 〈 0.05）. The Annexin V/PI double staining showed that the apoptosis rate increased induced by A549 cells exposed to drug-contained serum（20% ） after 24,48h and drug-contained serum（ 10% ） after 48h（ P 〈0.05）. The apoptosis rate of SMMC-7721 ceils exposed to drugcontained serum（ 10% ,20% ） after 24h and 48h also can be increased （ P 〈 0.05）. Conclusion： Serum containing Ermu particles can inhibit the growth of A549 and SMMC-7721 cells by inducing cell apoptosis.

关 键 词：二母颗粒 肺腺癌A549 肝癌SMMC-7721 凋亡 

分 类 号：R285[医药卫生—中药学]