高良姜素对人肝癌SMMC-7721细胞PI3K/AKT信号通路的影响  

Influence of galangin on the apoptosis of human hepatoma cells SMMC-7721 throughPI3K/AKT signal pathway

作　　者：刘影[1] 梅晰凡[1] 王晶[1] 张亚秋[1] 

机构地区：[1]辽宁医学院药学院,锦州121001

出　　处：《中药药理与临床》2014年第1期40-42,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁医学院科研基金资助项目(LYHX2012043)

摘　　要：目的:观察高良姜素对人肝癌SMMC-7721细胞PI3K/AKT信号通的影响。方法:采用MTT法检测高良姜素对人肝癌SMMC-7721细胞增殖的影响,流式细胞术检测细胞周期和细胞凋亡,Western印迹法检测PI3K/AKT信号通路相关蛋白及Caspase-9蛋白表达情况。结果:高良姜素抑制人肝癌SMMC-7721细胞增殖作用明显,且呈浓度和时间依赖性,顺铂和高良姜素(5.4、10.8、21.6、43.2、86.4μg/ml),72 h抑制率分别为69.4%、34.3%、44.9%、60.8%、73.9%、79.1%。流式细胞术检测结果显示高良姜素(10.8、21.6、43.2μg/ml)可使细胞周期阻滞于G1期。高良姜素(10.8、21.6、43.2μg/ml)处理24h凋亡率分别为9.8%、15.3%、18.6%。Western印迹法检测相关蛋白,药物处理组PI3K、AKT、P-AKT的表达受抑制,PTEN,Caspase-9蛋白的表达率随着药物浓度的增加而增加。结论:高良姜素可能通过抑制PI3K/AKT信号通路诱导人肝癌SMMC-772细胞凋亡。Objective： To investigate the influence of galangin on the proliferation and apeptosis of human hepatoma cells SMMC-7721 through PI3K/AKT signal pathway. Method： Proliferation effect of galangin on SMMC-7721 cell growth was detected by MTT assay. Cell cycle chan- ges were analyzed by flow cytometry （FCM）. The Western blot test was employed for evaluating influence of galangin protein expression in PI3K2AKT signal pathways of SMMC-7721. Result： Galangin inhibited obviously cell proliferation of SMMC-7721 in a concentration and time dependence manner. The inhibition rates of cisplatin and galangin （5.4,10.8,21.6,43.2,86.4μg/ml, 72 h） were 69.4%, 44.9%, 60.8%, 73.9%, 79.1% respectively. FCM showed that （ 10.8,21.6,43.2μg/ml） of galangin could arrest SMMC-7721 cells in G1 phase. The inhibition rates of galangin （ 10.8,21.6,43.2μg/ml, 24h） were 9.8%, 15.3%, 18.6% respectively. Western blot showed that protein expression PI3K, AKT, P-AKT were inhibited in the drug groups. The protein expression of PTEN and caspase-9 increased with the. drug concentration. Conclusion： Galangin can induce apoptosis of human hepatoma Cells SMMC-7721 through PL3K/AKT signal pathways.

关 键 词：高良姜素 人肝癌SMMC-7721细胞 PI3K-AKT信号通路 细胞凋亡 

分 类 号：R285[医药卫生—中药学]