七十味珍珠丸对局灶性脑缺血大鼠的神经保护作用  

Neural protective effect of Rannasangpei on local cerebral ischemia in rats

作　　者：甄丽芳[1] 罗远带[2] 黄福开[2] 胡贤达 邵杰[2] 刘亚丽[1] 尚颖[1] 杜文兵[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]北京藏医院,北京100029

出　　处：《中药药理与临床》2014年第1期90-92,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重大财政专项"藏医心脑血管病临床研究与药物研发";项目编号:1981020421

摘　　要：目的:探讨七十味珍珠丸对永久性大脑中动脉阻塞(pMCAO)大鼠的神经保护作用。方法:取SD大鼠用线栓法制备pMCAO模型。观察七十味珍珠丸对pMCAO大鼠神经功能损伤情况(Bederson评分和Rotarod test)、血清中神经元特异性烯醇化酶(NSE)及S-100β蛋白水平的影响。结果:与pMCAO模型组比较,七十味高剂量组(664mg/kg)显著降低Bederson评分,延长转棒潜伏期(P<0.01);与pMCAO模型组比较,七十味高剂量组(664mg/kg)、低剂量组(166mg/kg)中NSE和S-100β蛋白水平都有不同程度的下降(P<0.01)。结论:七十味珍珠丸可有效降低pMCAO大鼠血清中NSE、S-100β蛋白水平,改善其神经功能缺损,具有较好的神经保护作用,效应细胞既在神经元,又在神经胶质细胞。Objective：To explore the neural protective effect of Rannasangpei on the permanent middle cerebral artery occlusion （pMCAO） in rats. Methods：SD rats were madesin to focal cerebral ischemic model with the method of permanent middle artery occlusion. The effects of Rannasangpei on the degrees of neurological function deficit （ Boderson scores and Rotarod test were used ） in pMCAO rats and on the levels of S- 100β protein and Neuron-speciflc enolase （NSE） in their serum were observed. Results： 1. Bedcrson＇s scores and Botarod test： Compared with the pMCAO model group, Bederson＇s scores were significantly decreased in the high dose Rannasangpei groups （664mg/kg） and the dwell time of rats during rotarod test in the above groups was extended（P 〈0.01 ）. 2. NSE and S-100β protein levels： Compared with the pMCAO modal group, the S-100β protein and NSE contents in serum of the rats in the high（664mg/kg） and low（166mg/kg） dose Rannasangpei groups were decreased in different degrees （P 〈 0.01 ）. Conclusion： Rannasangpei which can lower the serum NSE and S-100β protein levels in pMCAO rats and improve the nerve function defect is protective on brain injury, the ceils which Rannasangpei can protect are both neurons and neuroglia cells.

关 键 词：七十味珍珠丸 局灶性脑缺血损伤 神经行为学 神经元特异性烯醇化酶(NSE) S-100Β蛋白 

分 类 号：R285[医药卫生—中药学]