参银复智制剂对血管性痴呆模型大鼠海马CA1区的改善作用及机制  

The improvement of Shenyinfuzhi preparation on hippocampus CA1 zone of vascular dementia model rats and mechanisms

作　　者：王琳[1] 马晓清[2] 郭云娣[1] 朱缨[1] 吴纪凯[1] 

机构地区：[1]苏州卫生职业技术学院,苏州215009 [2]香港浸会大学中医药学院

出　　处：《中药药理与临床》2014年第1期102-105,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：苏州卫生职业技术学院课题2011013"一种抗血管性痴呆的复方制剂的制备及质量标准";江苏省优秀骨干教师境外研修项目研究成果之一

摘　　要：目的:研究参银复智制剂对拟血管性痴呆(vascular dementia,VD)大鼠海马CA1区的改善作用及相关机制。方法:通过反复夹闭再通大鼠双侧颈总动脉制作拟血管性痴呆大鼠模型,按照1ml/100g体重灌胃给药,每天1次,连续30天,假手术组和模型组灌胃生理盐水,高、中、低剂量组分别灌胃2.32g,1.16g,0.58g生药/ml的参银复智混悬液1ml/100g,阳性药物组灌胃0.024mg成药/ml喜得镇混悬液1ml/100g。通过苏木素-伊红和刚果红染色比较使用大、中、小剂量参银复智制剂治疗后的海马CA1区细胞形态学改变和细胞数目及淀粉样蛋白沉淀情况,Western blot检测相关蛋白的表达。结果:模型组海马CA1区锥体细胞数明显减少,其顶树突缩短甚至消失,淀粉样蛋白沉积明显,应用参银复智制剂能减轻海马CA1区的损伤。参银复智制剂大、中剂量组的细胞数量和淀粉样蛋白沉淀与小剂量组相比有明显改善。Western blot检测发现,参银复智大、中剂量组可明显降低血管痴呆模型大鼠海马β-淀粉样蛋白(β-amyloid,Aβ)、β-淀粉样前体蛋白裂解酶1(β-site amyloid precursor protein cleaving enzyme 1,BACE1)、淀粉样前体蛋白(amyloid protein precursor,APP),但对早老素1(presenilin 1,PS1)表达没有明显影响;可明显提高模型大鼠海马神经内肽酶(neprilysin,NEP)和胰岛素降解酶(insulin-degrading enzyme,IDE)的表达。结论:参银复智制剂能发挥血管痴呆型大鼠的海马CA1区的保护作用,降低海马Aβ的沉积,这可能是其改善VD大鼠学习记忆障碍的重要机制。Objection： To observe the effects of Shenylnfuzhi preparation on protecting the cells in the CA1 area of hippocampus in the vascular dementia rats, VD rat models and to illustrate the possible mechanisms. Methods： Make model by repeated bilateral occlnsion of the eommunis carotis arteria. VD rat models were administrated orally with different dose of drug or saline a day for consecutive 30 days. Observe the number of cells and the area of amyloid plaque in the CA1 district of hippocampns through H-E and Congo red dying hippocampns, and determine the related protein by western blot. Results： the loss of pyramidal neurons , shortened apicaldendfite of neurons and increased amyloid plaque were revealed in hippocampal CA1 area in the VD model group, while the rats in the Shenyinfuzhi-treated groups have more cells and smaller amyloid plaque in the CA1 area of hippocampus than those in the VD model group. Western blot analysis showed that Shenyinfuzhi high dose group and medium dose group significantly （ P 〈 0. 05 ） reduced hippoeampal Aβdeposition, and the expressions of BACE1 and APP, but did not affect PSI expressions. Shenyinfuzhi high dose group and medium dose group also increase significantly （P 〈0. 05 或 P 〈 0. 01 ） NEP and IDE expression. Conclusion： Sbenyinfuzhi preparation could protect the cells in the CA1 area of hippocampns and reduce the deposit area of amyloid plaque in VD rats, which may be one of important mechanisms of Sbenyinfuzhi in improving learning and memory dysfunction of VD rats.

关 键 词：参银复智 血管性痴呆 海马CA1 

分 类 号：R285[医药卫生—中药学]