凉血通瘀方对出血性中风患者外周血细胞因子的影响  

Influence of Liangxu Tongyu Fang on expression of cytokines in the peripheral blood of patients with hemorrhagic stroke

作　　者：黄艳[1] 周肸[1] 赵凤鸣[1] 吴勉华[1] 周学平[1] 王明艳[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中药药理与临床》2014年第1期126-128,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家科技部973计划基金资助项目(2006CB504807);江苏省普通高校自然科学研究计划资助项目(10KJB360005)

摘　　要：目的:观察凉血通瘀方及其君药——大黄单体(大黄酸、大黄素)对出血性中风患者外周血中炎症相关细胞因子的影响,探讨凉血通瘀方治疗出血性中风患者的作用及机制。方法:采集出血性中风患者的全血,培养加药,放射性免疫法检测细胞上清中白介素-1β(IL-1β)、白介素-10(IL-10)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量,Western-blot法检测TNF-α、核因子-κB(NF-κB)、一氧化氮合成酶(iNOS)蛋白的表达水平。结果:凉血通瘀方(1g/ml)及大黄酸(25μmol/L)、大黄素(50μmol/L)可降低大鼠IL-1β、IL-6、TNF-α、NF-κB的表达,升高IL-10、iNOS的表达。结论:凉血通瘀方及大黄酸、大黄素降低炎症反应可能是其治疗出血性中风的作用机理之一。Objective：To observe the effect of liangxue tongyu fang and monomer（ rhein, emodin） on expression of cytokines in the peripheral blood of patients with hemorrhagic stroke and study the mechanism of liangxue tongyu fang in the treatment of hemorrhagic stroke. Methods： Periph- eral blood of patients with hemorrhagic stroke was collected. It was cultured and added drugs to. Supernatant IL-1β,IL-10, IL-6 ,TNF-α level was measured by radioimmunoassay. Expression of TNF-α, NF-κB,iNOS protein was measured by Western-blot. Results ：The results showed that Liangxue Tongyu Fang（1g/ml） , rhein（25umol/L） and emodin（50umol/L） can reduce the expression of IL-1β, IL-6, TNF-α, NF-κB level and increase expression of IL-10 ,iNOS. Conclusion： Liangxue Tongyu Fang, rhein and emodin reduce the inflammatory response and it is likely to be one of the the mechanism of the treatment of hemorrhagic stroke.

关 键 词：凉血通瘀方 大黄酸 大黄素 出血性中风 细胞因子 

分 类 号：R285[医药卫生—中药学]