肿节风总黄酮及迷迭香酸、落新妇苷对大鼠骨髓巨核细胞增殖的影响  

Effects of Flavonoids Sarcandrae and its ingredients rosmarinic acid and astilbin on proliferation of rat bone marrow megakaryocytes

作　　者：汤喜兰[1] 廖清花 鲍天冬 尚广彬[1] 李佳[1] 朱峰[1] 李冰涛[1] 张启云[1] 徐国良[1] 

机构地区：[1]江西中医药大学中医基础理论分化发展研究中心,南昌330004 [2]江西济民可信医药有限公司,南昌330096

出　　处：《中药药理与临床》2014年第2期47-49,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(81001662;81260653);江西省自然基金(2009GQY0109);江西省教育厅项目(GJJ11191)

摘　　要：目的:探讨肿节风总黄酮部位及其成分迷迭香酸、落新妇苷对大鼠骨髓巨核细胞增殖及血小板生成素(TPO)、转化生长因子-β1(TGF-β1)的影响。方法:采用兔抗大鼠血小板相关抗体(稀释倍数为128倍)建立大鼠骨髓巨核细胞增殖障碍模型,研究肿节风总黄酮部位500、250、125、62.5μg/ml,迷迭香酸500、250、125、62.5μg/ml及落新妇苷500、250、125、62.5μg/ml对大鼠骨髓巨核细胞增殖及培养上清液中TPO及TGF-β1含量的影响。结果:(1)肿节风总黄酮部位500、250、125μg/ml,迷迭香酸250、125μg/ml及落新妇苷500、250μg/ml均可显著促进大鼠骨髓巨核细胞的增殖;(2)肿节风总黄酮部位500、250、125、62.5μg/ml,迷迭香酸500、250μg/ml及落新妇苷500、250、62.5μg/ml均可显著降低培养体系中TPO的含量;肿节风总黄酮部位250、125、62.5μg/ml及落新妇苷500、125μg/ml均可显著增加培养体系中TGF-β1的含量,迷迭香酸对培养体系中TGF-β1的含量无明显影响。结论:肿节风总黄酮部位、迷迭香酸及落新妇苷均可以促进大鼠骨髓巨核细胞的增殖,迷迭香酸及落新妇苷是肿节风总黄酮部位促骨髓巨核细胞增殖的物质基础。Objective： To observe the effects of flavonoids sarcandrae and its ingredients（ rosmarinic acid and astilbin） on the proliferation and TPO,TGF-β1 levels of rat bone marrow megakaryocytes. Methods： The proliferation disorder model of rat bone marrow megakaryocytes was established by using rabbit anti-rat platelet associated antibody. The effects of flavonoids sarcandrae at the concentrations of 500,250,125and 62. 5 μg /ml,rosmarinic acid at the concentrations of 500,250,125 and 62. 5 μg /ml,and astilbin at the concentrations of 500,250,125 and 62. 5 μg /ml on the proliferation of rat bone marrow megakaryocytes and the levels of TPO and TGF-β1 in the culture supernatants were observed. Results：（ 1） Flavonoids sarcandrae at the concentrations of 500,250 and 125 μg /ml,rosmarinic acid at the concentrations of 250 and 125 μg /ml,and astilbin at the concentrations of 500 and 250 μg /ml can promote the proliferation of rat bone marrow megakaryocytes（ P ＆lt; 0. 01 or P ＆lt; 0. 05）;（ 2） Flavonoids sarcandrae at the concentrations of 500,250,125 and 62. 5 μg /ml,rosmarinic acid at the concentrations of 500 and 250 μg /ml,and astilbin at the concentrations of 500,250 and 62. 5 μg /ml can significantly decrease TPO level in the culture supernatant（ P ＆lt; 0. 01 or P ＆lt; 0. 05）; flavonoids sarcandrae at the concentrations of 250,125 and 62. 5 μg /ml,and astilbin at the concentrations of 500 and 125 μg /ml can significantly increase TGF-β1 level in the culture supernatant,but rosmarinic acid has no obvious effect. Conclusion： Flavonoids sarcandrae,rosmarinic acid and astilbin can promote proliferation of rat bone marrow megakaryocytes. Rrosmarinic acid and astilbin are the material basis of flavonoids sarcandrae that promote proliferation of rat bone marrow megakaryocytes.

关 键 词：肿节风 迷迭香酸 落新妇苷 增殖 骨髓巨核细胞 

分 类 号：R285[医药卫生—中药学]