两株鸭源H3N2亚型禽流感病毒分离株的分离与鉴定  

Isolation and identification of 2 H3N2 subtype avian influenza virus isolates

作　　者：关立峥[1,2] 李文辉[2] 王晶[2] 谭丹[2] 崔鹏飞[2] 卢昆鹏[2] 张芳[2] 邓国华[2] 陈化兰[2] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放试验室/兽医生物技术国家重点试验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第6期423-426,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技攻关项目(KJYJ-2013-01-01)

摘　　要：为了解我国H3亚型禽流感病毒(AIV)的生物学特性,本研究对鸭体内分离到的两株鸭源H3N2亚型AIV A/Duck/Hubei/S3176/2012(H3N2)(HuB/176/12)和A/Duck/Fujian/S1134/2011(H3N2)(FJ/134/11)进行了全基因组序列测定,并进行了SPF鸡和BALB/c小鼠的致病性试验。序列分析显示:HA裂解位点基序为339PEKQTR↓GLFG348,符合低致病性病毒株氨基酸序列特征。病毒内部基因来源较复杂,分离株与H1、H2、H5、H6、H7、H9、H12等多种亚型的低致病性AIV同源性较高,不同基因片段呈现明显的异源性。动物试验显示,病毒感染SPF鸡后两株病毒的排毒方式呈现明显差异,HuB/176/12仅通过咽喉向外排毒,而FJ/134/11能够通过咽喉和泄殖腔同时向外排毒,并且在鸡群中具有良好的水平传播能力,鸡感染病毒后多处脏器均能检测到病毒的存在。对BALB/c小鼠的致病性试验结果显示,病毒仅能在小鼠呼吸道中复制,不能够造成全身性系统感染。To investigate the prevalence of H3 subtype avian influenza virus （AIV） in China, two AIVs （HuB/176/12 and FJ/134/11） of H3N2 subtype were isolated from live-poultry markets during 2011 to 2012. The sequencing results showed that both isolates had a HA cleavage site of 339PEKQTR↓GLFG348, which was a characteristic of low pathogenesis AIV, and internal genes were highly homologous to H1, H2, H5, H6, H7, H9 and H12 subtype, respectively. In addition, the pathogenetic test showed that the HuB/176/12 was able to shedding through the respiratory only, but the FJ/134/11 AIV shedding through the respiratory and gastrointestinal tracts. In addition, the isolates had the ability to spread among chickens and the virus was restricted replication in trachea, kidney, fabricius, appendix and lung in the infected chickens. Experimental infection tests showed that the isolates were both low-pathogenic for mice which were only replicated in nasal shall and lung and caused a slight histopathogical lesions.

关 键 词：禽流感病毒 H3N2亚型 抗原性分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]