雷公藤多苷降低糖尿病肾病大鼠炎性细胞因子的表达  

Tripterygium wilfordii polyglycoside suppresses inflammatory cytokine expression in rats with diabetic nephropathy

作　　者：刘国玲[1] 沈永杰[1] 尤丽菊[1] 宋玮[1] 芦琨[1] 李宜川[1] 

机构地区：[1]商丘医学高等专科学校生物化学与分子生物学教研室,河南商丘476100

出　　处：《细胞与分子免疫学杂志》2014年第7期721-724,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河南省教育厅自然科学研究计划项目(2011C310003)

摘　　要：目的观察雷公藤多苷(TWP)对糖尿病肾病(DN)大鼠核因子κB(NF-κB)、C-C型趋化因子配体5(CCL5)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)等炎性细胞因子表达的影响。方法链脲佐菌素(STZ)腹腔注射建立DN大鼠模型。动物随机分为正常组、模型组、1.8 g/kg/d TWP治疗组。用药8周末,计算肾脏指数(KI)、全自动生化分析仪检测大鼠血糖(BG)、糖化血红蛋白(HbA1c)、血肌酐(Scr)、血尿素氮(BUN)等生化指标。ELISA检测大鼠血清超敏C反应蛋白(hs-CRP)和肾匀浆IL-6、TNF-α和CCL5的含量;HE染色观察大鼠肾脏病理学改变;免疫组织化学染色检测大鼠肾组织NF-κB的表达。结果与模型组相比,TWP可改善DN大鼠一般状况;明显降低DN大鼠空腹BG、HbA1c、KI、BUN及Scr水平,增加DN大鼠体质量(P<0.01);与模型组比较,TWP明显降低DN大鼠血清hs-CRP水平及肾匀浆中IL-6、TNF-α和CCL5的水平,明显下调DN大鼠肾组织NF-κB的表达(P<0.01),经TWP治疗后DN大鼠肾脏病理变化减轻。结论 TWP可降低糖尿病大鼠血清及肾脏炎性细胞因子表达,减轻肾脏病变。Objective To investigate the effects of Tripterygium wilfordii polyglycoside （TWP） on the expressions of nuclear factor kappa B （NF-KB）, chernokine （C-C motif） ligand 5 （CCL5）, tumor necrosis factor alpha （TNF-α）, interleukin-6 （IL-6） and other inflammatory factors in diabetic nephropathy （DN） rats. Methods The diabetic model was induced by a single intraperitoneal injection of streptozotocin （STZ）. The rats were randomly divided into three groups： normal control group, diabetic model group and TWP （1.8 g/kg · d） treatment group. Kidney index, blood glucose （BG）, glycosylated hemoglobin （HbAlc）, creatinine （Scr）, blood urea nitrogen （BUN） and other biochemical criterions were measured after eight-week treatment. The concentration of high sensitive C-reactive protein （hs-CRP） in serum and IL-6, TNF-α, CCL5 in kidney homogenate were detected by ELISA. The pathological changes of kidney were observed by HE staining and the expression of NF-KB in kidney was tested by immunohistochemistry. Results Compared with the model group, TWP improved evidently the general situation of rats. The levels of BG, HbAlc, kidney index, Scr and BUN were reduced significantly （P 〈 0.01 ） and the body mass was elevated significantly （P 〈 0.01 ）. Additionally, the content of hs-CRP in serum, the levels of IL-6, TNF-α, and CCL5 in kidney homogenate and the expression of NF-κB in kidney decreased obviously in TWP group compared with the model group（ P 〈 0.01 ）. The pathological changes of kidney were also relieved by TWP treatment. Conclusion TWP treatment could reduce the expressions of inflammation cytokines in serum and kidney and relieve the nephropathy in diabetic rats.

关 键 词：雷公藤多苷 糖尿病肾病 炎症损伤 核转录因子-ΚB C-C型趋化因子配体5 

分 类 号：R392-33[医药卫生—免疫学] R284.2[医药卫生—基础医学]