血浆可溶型B7-H3可作为乙型肝炎病毒相关性肝癌辅助诊断标志物  

Plasma sB7-H3 serves as an assistant diagnostic marker for hepatitis B virus-associated hepatocellular carcinoma

作　　者：许文[1] 李丰衣[1] 余灵祥[1] 刘佳[1] 何旭[1] 范荣[1] 陈威巍[1] 

机构地区：[1]解放军第302医院感染性疾病诊疗与研究中心,北京100039

出　　处：《细胞与分子免疫学杂志》2014年第7期744-747,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家科技重大专项(2012ZX10005-006)

摘　　要：目的研究血浆中可溶型B7-H3(sB7-H3)与乙型肝炎病毒相关性肝癌(HBV-HCC)的关系。方法选择在解放军第302医院住院治疗的HBV-HCC患者23例及健康体检者(HC)15例,采用ELISA检测外周血浆中sB7-H3含量;设计sB7-H3基因特异性引物,采用相对实时定量PCR(qRT-PCR)方法检测外周血单个核细胞(PBMC)、癌和癌旁组织中sB7-H3 mRNA的表达;采用免疫组织化学染色检测癌和癌旁组织中的膜型B7-H3(mB7-H3)表达。结果 HBV-HCC患者血浆中sB7-H3含量显著高于健康对照组(P<0.05),但HCC患者和健康对照组PBMC中sB7-H3的mRNA表达水平无显著差异(P>0.05);而肝癌切除术后HBV-HCC患者血浆中sB7-H3含量显著下降(P<0.05),同时HBV-HCC患者癌和癌旁组织中表达的sB7-H3 mRNA比其PBMC显著升高,分别可达到PBMC中sB7-H3 mRNA表达量的60倍和1 800倍;癌旁组织中的sB7-H3 mRNA水平高于自身癌组织近30倍;癌旁组织中mB7-H3表达显著高于癌组织。结论肝癌及癌旁组织是外周血中sB7-H3的主要来源之一,sB7-H3可作为HBV-HCC的辅助诊断标志物。Objective To investigate the relationship between the expression of soluble B7-H3 （sBT-H3） in the plasma and hepatitis virus B-associated hepatocellular carcinoma （HBV-HCC）. Methods Twenty-three patients with HBV-HCC and 15 healthy donors were enrolled in this study. The level of sB7-H3 in the plasma was detected by ELISA. The expression of sB7-H3 mRNA in peripheral blood monocytes （ PBMCs）, tumor tissues and peri-tumor normal tissues were determined by relative real-time quantitative PCR （qRT-PCR）. The level of membrane BT-H3 （mBT-H3） expressed in tumor tissues and peri-tumor normal tissues were detected by immunohistochemical staining. Results The level of plasma sBT-H3 in the HBV-HCC patients was significantly higher than that in the healthy donors （ P 〈 0.05）. However, the level of sB7-H3 mRNA in PBMCs was not significantly different between the HBV-HCC patients and healthy donors （ P 〉 0. 05）. The level of plasma sB7-H3 dropped significantly after the resection of liver tumors （ P 〈0.05）. The qRT-PCR showed the expression of sBT-H3 mRNA in tumor tissues and peri-tumor normal tissues were about 60 and 1800 times higher than those in PBMCs of the corresponding HBV-HCC patients, respectively, and the expression of sB7-H3 mRNA in peri-tumor normal tissues was about 30 times higher than that in tumor tissues. Immunohistochemical staining revealed that the expression of mB7-H3 in peri-tumor normal tissues was higher than that in tumor tissues. Conclusion sB7-H3 in the plasma of HBV-HCC patients is mainly generated from tumor and peri-tumor normal tissues, and it can serve as an assistant diagnostic marker for HBV-HCC.

关 键 词：乙型肝炎病毒 肝细胞癌 可溶性B7-H3 生物标志物 

分 类 号：R392.33[医药卫生—免疫学] R735.7[医药卫生—基础医学]