间充质干细胞通过诱导髓系来源CD11b^+Gr1^+细胞的抑制功能促进小鼠乳腺癌进展  

Mesenchymal stem cells promote mouse breast tumor progression by inducing the suppressive function of myeloid-derived CD11b^+Gr1^+ cells

作　　者：胡晓玉 罗悦晨[1] 周玉山 赵丹[1] 吴万通 韩忠朝[1] 冯晓明[1] 

机构地区：[1]中国医学科学院北京协和医学院血液病医院,血液学研究所,天津300020

出　　处：《细胞与分子免疫学杂志》2014年第8期785-788,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重大科学研究计划(2013CB966904);国家自然科学基金(81273217);天津市应用基础及前沿技术研究计划(12JCYBJC32800);高等学校博士学科点专项科研基金(20121106120037)

摘　　要：目的探讨间充质干细胞(MSC)诱导骨髓来源的CD11b+Gr1+细胞免疫抑制功能对肿瘤的影响。方法将骨髓中CD11b+Gr1+细胞与MSC共培养后,通过流式细胞术测定CD11b+Gr1+细胞免疫表型,通过T细胞抑制性实验分析T细胞活化情况,采用小鼠4T1乳腺癌模型观察MSC通过CD11b+Gr1+细胞对肿瘤生长的影响。结果 MSC能够促进骨髓中CD11b+Gr1+细胞的存活和生成,同时可诱导正常小鼠骨髓中CD11b+Gr1+细胞对T细胞的抑制作用。动物实验结果显示,MSC处理后的骨髓CD11b+Gr1+细胞可以促进肿瘤的生长,加速小鼠的死亡。结论 MSC可以通过促进骨髓来源的CD11b+Gr1+细胞的抑制作用加速肿瘤的生长进程。Objective To investigate the role of mesenchymal stem cells （MSCs） in tumor progression by inducing immuno-suppressive function of myeloid-derived CD11b＋Gr1＋ cells （BM-CD11b＋Gr1＋ cells）. Methods After co-cultured with MSCs, the immunophenotypes of BM-CD11 b ＊ Grl ＋ cells were tested by flow cytometry. The influence of MSCs on CD11b＋Gr1＋ cells was evaluated by T cell proliferation assay after cultured with or without MSCs. The mouse 4T1 breast tumor model was established to explore the effect on tumor growth. Results MSCs promoted the survival of CD11b＋Gr1＋ cells and induced the generation of CD11b＋Gr1＋ cells from CD11b＋Gr1＋ cells sorted from bone marrow. MSCs also enhanced the ability of BM-CD11b＋Gr1＋ cells to suppress T cell proliferation and activation. CD11b＋Gr1＋ cells after co-cultured with MSCs promoted 4T1 tumor growth and accelerated death of tumor-bearing mice. Conclusion MSCs can promote tumor progression by inducing suppressive function of myeloid-derived CD11b＋Gr1＋ cells from bone marrow.

关 键 词：间充质干细胞 CD11b+Gr1+细胞 肿瘤免疫 

分 类 号：R392.11[医药卫生—免疫学] Q279[医药卫生—基础医学]