黄芩素通过抑制ERK通路下调HeLa细胞中基质金属蛋白酶的表达  

Baicalein induces HeLa cell growth inhibition by down-regulation of matrix metalloproteinases and activating extracellular signal-regulated kinase

作　　者：喻小兰[1,2] 卢科莲 夏纪毅[4] 毛熙光[5] 

机构地区：[1]泸州医学院 [2]泸州市龙马潭区妇幼保健院,四川泸州646000 [3]泸州市妇幼保健院 [4]泸州医学院药物与功能性食品研究中心 [5]泸州医学院附属医院妇产科

出　　处：《细胞与分子免疫学杂志》2014年第8期798-801,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省科技厅课题基金(09032);四川省卫生厅课题基金(12193)

摘　　要：目的观察不同浓度黄芩素处理宫颈癌细胞后,细胞外信号调节激酶1/2(ERK1/2)与基质金属蛋白酶2(MMP-2)、MMP-9 mRNA及蛋白水平表达的变化。方法体外培养HeLa细胞株,分为空白对照组、100μmol/L、200μmol/L黄芩素处理组,培养24 h。明胶酶谱法检测上清液中MMP-2、MMP-9的活性变化。反转录PCR法检测ERK1/2及MMP-2、MMP-9的mRNA水平的表达变化。Western blot法检测ERK1/2、p-ERK1/2及MMP-2、MMP-9的蛋白水平的表达变化。结果黄芩素处理24 h后,与空白对照组相比较,MMP-2、MMP-9的活性变化逐渐降低,且呈浓度依赖性(P<0.05)。ERK1/2、p-ERK1/2、MMP-2、MMP-9的mRNA及蛋白表达随着黄芩素浓度的增强逐渐降低(P<0.05)。结论黄芩素通过激活ERK1/2信号通路下调MMP-2、MMP-9的表达。Objective To observe the changes of mRNA and protein expressions of extracellular signal-regulated kinase 1/2 （ERK1/2） and matrix metalloproteinase 2 （ MMP-2）, MMP-9 in cervical cancer cells after stimulated by baicalein at different concentrations. Methods HeLa cells cultured in vitro were exposed to baicalein （0, 100 and 200 iamol/L） for 2.4 hours. The activity of MMP-2 and MMP-9 was measured by gelatin zymography, mRNA expressions of ERK1/2, MMP-2, MMP-9 were determined by reverse transcription PCR （RT-PCR） and protein expressions of ERK1/2, MMP-2, MMP-9 were detected by Western blotting. Results Compared with the control group, baicalein significantly decreased the activity of MMP-2 and MMP-9 in a concentration-dependent manner （ P 〈 0.05）. The mRNA and protein expressions of ERK1/2, p-ERKI/2, MMP-2, MMP-9 also significantly decreased （ P 〈 0.05）. Conclusion Baicalein can activate ERK1/2 signaling pathway and decreasing the activity of MMP-2 and MMP-9.

关 键 词：黄芩素 宫颈癌细胞 MMP-2 MMP-9 细胞外信号调节激酶1 2 

分 类 号：R285.5[医药卫生—中药学] R737.33[医药卫生—中医学]