细胞表面黏附分子介导机械性牵张力诱导的小鼠颈总动脉HL-60细胞黏附  

Cell surface adhesion molecules mediated stretch-induced HL-60 cell adhesion to mouse common carotid arteries

作　　者：霍迎庆 蒋蓓蓓[1] 韩晓帆[1] 罗金才[1] 

机构地区：[1]北京大学分子医学研究所血管生物学研究室,北京100871

出　　处：《细胞与分子免疫学杂志》2014年第8期802-805,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：北京大学985专项基金(985II/56210405)

摘　　要：目的比较血管内皮细胞表面黏附分子-1(VCAM-1)及P-选择素在机械性牵张力诱导HL-60细胞向小鼠颈总动脉内皮细胞黏附中的作用。方法使用分离的小鼠颈总动脉血管在一定压力下灌流HL-60细胞,使之与血管内皮相互作用后洗去未黏附细胞,镜下统计每个视野中黏附的细胞数。首先观察不同强度牵张力刺激对于HL-60细胞向血管内皮细胞黏附的影响。再利用针对VCAM-1、P-选择素的特异性中和抗体以及非特异性IgG预处理血管条,比较VCAM-1和P-选择素在牵张力引起的HL-60细胞黏附中的差别情况。结果随着施加在颈动脉的静水压增大,HL-60细胞黏附逐渐增多,说明牵张力引起的HL-60细胞黏附是强度依赖性增加的。非特异IgG预处理血管条后,黏附的HL-60细胞数目无明显变化。然而与此对照,VCAM-1与P-选择素预处理组都导致了黏附的HL-60细胞显著减少(P<0.05),但2组间差异无统计学意义(P>0.05)。结论牵张引起的颈总动脉HL-60细胞黏附呈现压力强度依赖性关系;VCAM-1与P-选择素在机械性牵张力诱导小鼠颈总动脉HL-60细胞黏附中起一定作用。Objective To compare the roles of vascular cell adhesion molecular-I （VCAM-1） and P-selectin in stretch-induced HL-00 cell adhesion to mouse common carotid arteries. Methods After the isolated mouse common carotid arteries were perfused with HL-60 cells, the unbound leukocytes were washed away and the number of adherent cells in every single field was counted under a microscope. We first observed the effect of stretch on HL-60 cell adhesion to the endothelium of arteries under different magnitudes of luminal hydrostatic pressure. Then, the roles of VCAM-1 and P-selectin in stretch-induced HL-60 cell adhesion to mouse common carotid arteries were compared by the pretreatment of neutralizing antibodies against VCAM-1, P-selectin or isotype-matched control antibody, respectively. Results The increase in luminal hydrostatic pressure of carotid arteries led to the increase of the number of HL-60 adhesion cells, suggesting that stretch induced HL-60 cell adhesion in a magnitude-dependent manner. The pretreatment with the isotype-matched control antibody failed to induce a significant change of the number of HL-60 adhesion cells. In contrast, the pretreatment of both VCAM-1 and P-selectin neutralizing antibodies dramatically reduced the number of adherent leukocytes （ P 〈 0. 05）, although no statistical significance was observed between the two groups. Conclusion Stretch can induce HL-60 cell adhesion to carotid arteries in a magnitude-dependent manner. VCAM-1 and P-selectin play some roles in mechanical stretch-induced HL-60 cell adhesion to mouse common carotid arteries.

关 键 词：血管内皮黏附分子-1 P-选择素 牵张力 HL-60细胞黏附 小鼠颈总动脉 

分 类 号：R392.32[医药卫生—免疫学] Q274[医药卫生—基础医学]