高糖对原代成骨细胞增殖分化的影响  

Effects of high glucose on the proliferation and differentiation of primary osteoblasts

作　　者：董永辉[1] 徐飞[1] 郭风劲[1] 陈安民[1] 杜宇[1] 黄仕龙[1] 

机构地区：[1]华中科技大学同济医院附属同济医院骨科,武汉430030

出　　处：《中华老年医学杂志》2014年第6期665-667,共3页Chinese Journal of Geriatrics

摘　　要：目的观察不同浓度葡萄糖对原代成骨细胞增殖和细胞分化的影响。方法取新生小鼠颅骨进行原代培养。经碱性磷酸酶染色和VonKossa染色检测鉴定为成骨细胞。用不同浓度葡萄糖（5．5、15．5、25．5mmol／L）培养基培养细胞，观察不同葡萄糖浓度下成骨细胞的细胞增殖、碱性磷酸酶（ALP）染色，Real—timePCR检测转录因子Runx2、成骨标志物ALP、骨钙素基因的表达。结果随着葡萄糖浓度的升高成骨细胞的增殖能力逐渐减弱。培养7d，与5．5mmol／L正常糖组相比，15．5mmol／L组和25．5mmol／L组ALP染色颜色逐渐变浅。PCR结果显示Runx2 mRNA表达分别下降了（36．7±6．2）％和（38．3±2．2）％，骨钙素mRNA表达分别下降了（26．7±7．2）％和（40．4±4．3）％；ALPmRNA在15．5mmol／L组下降了（33．3±10．2）％，在25．5mmol／L组增高了（50．8±10．4）％（均P〈0．05）。结论高糖能抑制成骨细胞的增殖，抑制其向成熟成骨细胞分化，这可能是糖尿病性骨质疏松的机制之一。Objective To observe the effects of different concentrations of glucose on the proliferation and differentiation of primary osteoblasts. Methods The identification of mouse primary osteoblasts was performed by alkaline phosphatase （ALP） staining and Von Kossa staining. Treating osteoblasts with different dose of glucose （5.5, 15.5, 25.5 mmol/L）, the osteoblasts proliferation,ALP staining, and Runx2 , OB markers ALP and OCN mRNA expression were observed. Real-time PCR was used for the determination of Runx2, OB markers ALP and OCN mRNA expression. Results With the increasing glucose concentrations, the osteoblasts cell proliferation was decreased. Compared with 5.5 mmol/L normal glucose, the ALP staining in 15.5 mmol/L group and 25.5 mmol/L group were decreased. The expressions were decreased by （36.7 ± 6.2）% and （38.3±2.2）% in Runx2 mRNA, （26.7±7.2）% and （40.4±4.3）% in OCN mRNA respectively. ALP in 15.5 mmol/L group was reduced by （33.3 ± 10.2）%, but increased by（50.8 ±10.4） % in 15.5 mmol/L group. Conclusions High glucose may decrease osteoblasts proliferation and activity, which may be one of the key pathogenesis factors of diabetic osteoporosis.

关 键 词：葡萄糖 成骨细胞 细胞增殖 细胞分化 

分 类 号：R587.1[医药卫生—内分泌]