川芎嗪对咖啡因引起的PC12细胞损伤的保护作用  

Protective Effect of Tetramethylpyrazine on Caffeine-induced PC12 Cell Injury

作　　者：王佳[1] 高峰[1] 张春兵[1] 

机构地区：[1]江苏省中医院检验科,南京210029

出　　处：《医药导报》2014年第6期695-698,共4页Herald of Medicine

基　　金：江苏省自然科学基金资助项目(BE2010768);国家自然科学基金资助项目(81171659)

摘　　要：目的分析川芎嗪对咖啡因引起大鼠肾上腺嗜铬细胞瘤克隆化细胞株PC12细胞损伤的保护作用,探讨川芎嗪治疗脑缺血-再灌注损伤的机制。方法制备咖啡因细胞损伤模型,通过CCK-8法活细胞检测、流式细胞术线粒体膜电位测定、Western-blot检测高迁移率族蛋白B1(HMGB1)、酶联免疫吸附测定(ELISA)检测氧化应激指标观察咖啡因的毒性及川芎嗪的保护作用。结果川芎嗪预处理后,PC12细胞的存活数显著提高,细胞线粒体膜电位提高,HMGB1表达显著降低,超氧化物歧化酶(SOD)上调,乳酸脱氢酶(LDH)和丙二醛(MDA)下调,谷胱甘肽(GSH)升高。结论川芎嗪对咖啡因引起的PC12细胞损伤有显著的保护作用,其保护作用可能与川芎嗪抑制细胞凋亡、调节炎症性递质表达水平及氧化应激反应相关。Objective To analyze whether tetramethylpyrazine could protect PC12 cells from injuries induced by caffeine, and to explore the mechanism of tetramethyipyrazine in the treatment of cerebral ischemia-reperfusion injury. Methods Caffeine was added to induce apoptosis of PC12 cells. Cytotoxicity was detected by CCK-8 assay. The electric potential of mitochondrial membrane was determined by flow cytometry. HMGB1 was detected by Western blotting. Oxidative stress was detected by ELISA. We observed toxicity of caffeine and the protective effects of tetramethylpyrazine. Results After the pre- treatment,tetramethylpyrazine significantly improved PC12 cell survival. Mitochondrial membrane potential was increased, the expression of HMGB1 decreased, SOD increased, LDH and MDA decreased, and GSH elevated. Conclnsion Tetramethylpyrazine exerts a significant protective effect on PC12 cell injury caused by caffeine. The protective effect may be related to inhibition of apoptosis and regulation of the expression level of mediators involved in inflammation and oxidative stress.

关 键 词：川芎嗪 PC12细胞 损伤 脑缺血-再灌注 

分 类 号：R965[医药卫生—药理学]