青海大黄油菜粒色性状分子标记的开发和图谱整合  

Development of Molecular Markers and Map Integration for Seed Color Traits in Dahuang Rape(Brassica rapa L.)

作　　者：赵会彦[1] 肖麓[1] 赵志[1] 杜德志[1] 

机构地区：[1]青海大学农林科学院春油菜研究所/青海省春油菜遗传改良重点实验室/青海省高原作物种质资源创新与利用国家重点实验室培育基地,青海西宁810016

出　　处：《作物学报》2014年第6期965-972,共8页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2011AA10A104);国家重点基础研究发展计划(973计划)项目(2012CB723007);国家现代农业产业技术体系建设专项(CARS-13);国家自然科学基金项目(31060196)资助

摘　　要：利用青海大黄油菜和褐籽白菜型油菜09A-126构建BC4和F2分离群体, 结合AFLP与群体分离分析法（bulked segregant analysis, BSA）筛选引物, 获得5个与黄籽基因Brsc1紧密连锁的分子标记Y11-Y15。5个AFLP特异片段的序列, 均与白菜型油菜的A9染色体部分序列表现同源。将5个AFLP标记成功转化为5个SCAR标记（SC11-SC15）。利用目标基因所在染色体区段序列筛选到7个与目标基因紧密连锁的SSR标记（BrID10607、KS10760、B089L03-3和A1-A4）。利用SCAR和SSR标记扫描F2群体中部分单株, 发现SC14和A1为共显性标记。用BC4群体将Brsc1定位在标记Y06和A4之间1.7 Mb的区间内, 遗传距离分别为0.115 cM和0.98 cM。标记Y05和Y12与Brsc1共分离。本研究为黄籽油菜分子标记辅助选择育种体系的建立及目标基因的进一步精细定位和图位克隆奠定了基础。A BC4 population and a F2 population, derived from the cross between Dahuang and 09A-126 （brown seed, B. rapa）, were constructed. AFLP （amplified fragment length polymorphism） methodology and bulked segregant analysis （BSA） were used to get five AFLP markers closely linked to yellow-seeded gene Brsc1, termed Y11–Y15 respectively. Five AFLP specific fragments were homologue with some sequences on chromosome A09 of Brassica rapa, which we converted into five SCAR markers, termed SC11–SC15. Seven SSR markers, BrID10607, KS10760, B089L03-3, A1–A4, tightly linked to Brsc1 were developed in the region of chromosome where Brsc1 was located. With five SCAR markers and seven SSR markers used for genotyping in F2 population, SC14 and A1 were confirmed as co-dominant markers. Using BC4 population, Brsc1 was located in the region of 1.7 Mb between Y06 and A04 on chromosome A9 with genetic distances of 0.115 cM and 0.98 cM. Y05 and Y12 co-segregated with Brsc1. The results were useful for developing yellow-seeded rapeseed lines by marker-assisted selection （MAS）, and also laying the foundation for fine mapping and map-based cloning of Brsc1.

关 键 词：白菜型油菜 黄籽 分子标记 遗传图谱 物理图谱 

分 类 号：S565.4[农业科学—作物学]