蟾酥逆转人白血病K562/DOX细胞多药耐药的实验研究  

Experimental Studies on Venenum Bufonis in Reversing Multidrug Resistance of Human Leukemia Cell Line K562/DOX

作　　者：王珏莲 古学奎[2] 刘安平[2] 陈志雄[2] 蓝海[2] 

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中药新药与临床药理》2014年第3期280-283,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省中医药管理局资助项目(20121216);广东省科技发展计划资助项目(2012b03180019)

摘　　要：目的探讨传统中药蟾酥逆转人白血病K562/DOX细胞多药耐药性(MDR)的作用。方法采用中药血清药理学方法制备蟾酥含药血清,以MTT法分别检测蟾酥含药血清联合柔红霉素,或单用蟾酥含药血清处理后,不同时段作用于K562/DOX细胞的抑制率;实时荧光定量PCR法检测蟾酥含药血清对K562/DOX细胞的Bcl-2 mRNA表达。结果与空白对照组比较,蟾酥对K562/DOX细胞均有抑制作用;蟾酥组Bcl-2 mRNA表达降低。结论蟾酥可能通过下调MDR1表达水平进而逆转K562/DOX细胞的耐药性。Objective To explore the effect of Venenum Bufonis on reversing the multidrug resistance of human leukemia cell line K562/DOX. Methods Serum containing Venenum Bufonis was prepared firstly, and then was applied alone or together with Daunorubicin for the culture of human leukemia cell line K562/DOX. After treatment for some time, the inhibition ratio of K562/DOX was detected by MTT assay, and Bcl-2 mRNA expression in K562/DOX was examined by real-time fluorescent quantitation PCR. Results In comparison1 with the control group, Venenum Bufonis had an inhibition on K562/DOX and decreased the Bcl-2 mRNA expression. Conclusion Venenum Bufonis can down-regulate the MDR1 expression level, and thus reverse the multidrug resistance of hmnan leukemia cell line K562/DOX.

关 键 词：K562 DOX细胞 蟾酥 BCL-2基因 

分 类 号：R285.5[医药卫生—中药学]