巴戟天环烯醚萜苷类及蒽醌类成分HPLC指纹图谱研究  

Studies on HPLC Fingerprint of Iridoid and Anthraquinone Components of Radix Morindae Officinalis

作　　者：高新开 叶家宏[2] 曹子丰 魏刚[2] 林伟斌 吴小莉 

机构地区：[1]无限极(中国)有限公司,广东江门529156 [2]广州中医药大学,广东广州510006

出　　处：《中药新药与临床药理》2014年第3期315-318,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立巴戟天环烯醚萜苷类和蒽醌类成分HPLC指纹图谱。方法采用ZOBAX SB Aq(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸溶液(梯度洗脱);检测波长分别为235,280 nm;柱温为30℃;流速为1.0 mL·min-1。结果标示出巴戟天环烯醚萜苷类和蒽醌类成分指纹图谱具有8个和7个共有峰。结论市售巴戟天药材质量较稳定,本研究为巴戟天的质量控制提供了方法依据。Objective To establish HPLC fingerprint of iridoid and anthraquinone components of Radix Morindae Officinalis. Methods Zorbax SB Aq （250 mm x 4.6 mm, 5 μm） column was used with acetonitrile-0.1% phosphoric acid solution as the mobile phase by gradient elution, the flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃. The detection wavelength was set at 235 nm and 280 nm respectively. Results Eight characteristic peaks of iridoid components and 7 characteristic peaks of anthraquinone components were separated from 11 batches of Radix Morindae Officinatis. Conclusion The quality of Radix Morindae Officinalis in the market is stable, and the results will provide a reference for the quality control of Radix Morindae Officinalis.

关 键 词：巴戟天 环烯醚萜苷类 蒽醌类 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]