维生素A缺乏对大鼠脂质过氧化和抗氧化系统的影响  

EFFECT OF VITAMIN A DEFICIENCY ON LIPID PEROXIDATION AND ANTIOXIDATION SYSTEM IN RATS

作　　者：李英哲[1] 黄连珍[1] 周丽玲[1] 

机构地区：[1]同济医科大学营养与食品卫生学教研室,湖北武汉430030

出　　处：《营养学报》2001年第1期1-5,共5页Acta Nutrimenta Sinica

基　　金："九五"国家科技攻关课题!(No.96 -90 4-0 6 -0 6)

摘　　要：目的 :　研究维生素 A(VA)缺乏对 3w龄大鼠的脂质过氧化和抗氧化系统的影响。方法 :　健康 Wistar雄性大鼠 33只 ,按体重随机分为 A(VA完全缺乏组 ) ,B(VA轻度缺乏组 ) ,C(VA正常对照组 )三组。每组 1 1只 ,实验期为 84d。观察指标有 :血清 VA,血清、肝脏及脑组织的超氧物岐化酶 (SOD)活性 ,全血、肝脏及脑组织的谷胱甘肽过氧化物酶 (GSH- Px)活性 ,血清、肝脏及脑组织的丙二醛 (MDA)含量 ,并采用电子自旋共振 (electron spin resonance,ESR)和自旋捕捉技术直接测定大鼠肝组织中的氧自由基。结果 :　 A、B组大鼠血清、肝脏及脑组织 SOD活性明显下降 ,全血、肝脏及脑组织 GSH- Px活性明显降低 ,血清、肝脏及脑组织 MDA含量显著升高 ,ESR图谱出现了 N- H偶合的三组双峰波。结论 :　 VA完全或轻度缺乏均可以使大鼠脂质过氧化反应明显增强 。Objective: To study the effect of vitamin A deficiency on lipid peroxidation and antioxidation system in rats. [WT5FZ]Methods: According to body weight, 33 Wistar male rats were randomly divided into 3 groups, A (Vitamin A complete deficiency group), B (Vitamin A low grade deficiency group), C (Vitamin A normal control group). The study lasted 84 days. The serum vitamin A, serum, liver and brain SOD activities, blood, liver and brain GSH Px activities, serum, liver and brain MDA were studied. Spin trapping techniques combined with ESR were used to capture and detect free radicals generated in liver. [WT5FZ]Results: In vitamin A completely deficient group and vitamin A low grade deficient group, SOD activities of serum, liver and brain, and the GSH Px activities of blood, liver and brain decreased significantly, while LPO contents of serum, liver and serum increased markedly, and typical free radicals peaks in liver were indicated by ESR spectra. [WT5FZ]Conclusion: Vitamin A deficiency results in decrease of antioxidation capacity and increase of lipid peroxidation.

关 键 词：维生素A缺乏 脂质过氧化 抗氧化系统 电子自旋共振技术 

分 类 号：R965[医药卫生—药理学]