外源核酸促电离辐射损伤的小鼠肠腺细胞修复的相关基因克隆  

Cloning of genes related with exogenous nucleic acids improving the repair of intestinal epithelium after γ-irradiation in mice

作　　者：崔大祥[1] 曾桂英[2] 王枫[1] 郭晏海[1] 徐俊荣[1] 田芙蓉[2] 闫小君[1] 任东青[2] 苏成芝[1] 

机构地区：[1]第四军医大学全军基因诊断技术研究所,陕西西安710033 [2]第四军医大学预防医学系放射医学教研室,陕西西安710033

出　　处：《第四军医大学学报》2001年第5期403-406,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 !(3 8970 2 79);"2 11"工程基金项目! (98X2 0 7)

摘　　要：目的　克隆外源核酸促电离辐射损伤的小鼠肠腺细胞修复的相关基因 .方法　建立 BAL B/ c小鼠电离辐射后给予外源 RNA与生理盐水治疗的 6 ,12 ,及 2 4h,4和 8d的模型 ,采集空肠组织标本后采用消减杂交基础上的 L D- PCR技术 ,获取与受照小鼠肠腺损伤修复相关的基因克隆 ,对其进行全自动测序与 Gene Bank检索 ,新基因递交给基因库 .结果　获取了 90个与肠腺修复相关的基因 ,杂交证实在核酸治疗组与生理盐水治疗组之间呈差异表达 ,其中 18个是新基因 ,Gene Bank接受号为 AF2 40 16 4- 2 40 181.结论　克隆了AIM To explore the molecular mechanism of exogenous nucleic acids improving repair of radiation damaged crypt. METHODS 45 of the mice being irradiated by γ ray were treated with small intestinal RNA as test group, whose small intestinal specimens were collected at 6, 12 and 24 h, 4 d, 8 d after treatment respectively; 45 of the mice being irradiated by γ ray were treated with physiological saline as control group, whose small intestinal specimens were collected. Then genes expressed in test group higher than in control group, were obtained by using LD PCR based on subtractive hybridization. After that, these genes were cloned into T vectors, and then were sequenced. The obtained sequences were searched for GeneBank. RESULTS ①90 of clones associated with repair of radiation damaged crypt were obtained; ②RNA dot blot confirmed that 90 of clones were differentially expressed between test group and control group; ③By searching GeneBank, 18 of 90 genes were new sequences,GeneBank accept number were AF240164 AF240181. CONCLUSION Eighteen genes have been cloned.

关 键 词：小鼠 电离辐射 肠腺细胞 基因表达 基因克隆 外源核酸 

分 类 号：R977[医药卫生—药品] Q691.5[医药卫生—药学]